Hanna Sarah M, Willet Alaina H, Gould Kathleen L
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, US.
MicroPubl Biol. 2025 Apr 28;2025. doi: 10.17912/micropub.biology.001595. eCollection 2025.
Mob1 is the regulatory subunit of the protein kinase Sid2 . The Sid2- Mob1 complex is the most downstream acting component of the septation initiation network (SIN). In the absence of functional Mob1 , cells fail cytokinesis and become multinucleate. Here we characterize a set of temperature-sensitive alleles by identifying the mutations within each allele, characterizing the extent of their growth defects, and visualizing the cell defects. Based on structural modeling, we hypothesize that the Mob1 mutations interfere with Mob1 stability and its ability to bind the N-terminal regulatory region of Sid2 .
Mob1是蛋白激酶Sid2的调节亚基。Sid2-Mob1复合物是隔膜起始网络（SIN）中最下游起作用的组分。在缺乏功能性Mob1的情况下，细胞无法进行胞质分裂并变成多核细胞。在这里，我们通过鉴定每个等位基因内的突变、表征其生长缺陷的程度以及观察细胞缺陷来表征一组温度敏感等位基因。基于结构建模，我们推测Mob1突变会干扰Mob1的稳定性及其结合Sid2 N端调节区域的能力。
