Inouhe M, Inada G, Thomas B R, Nevins D J
Department of Biology and Earth Sciences, Faculty of Science, Ehime University, Matsayama, Ehime, Japan.
Int J Biol Macromol. 2000 Apr 12;27(2):151-6. doi: 10.1016/s0141-8130(00)00111-2.
Exo- and endo-glucanases mediate specific degradation of cell wall (1,3)(1,4)-beta-D-glucans and these enzymes have been related to auxin-mediated growth and development of cereal coleoptiles. However, their distribution and functions have not been well established in other tissues. In this study the glucanase activities and cell wall autolytic activities of different maize organs were determined. Autolysis assays serve to evaluate the hydrolysis of cell wall polymers in situ by measuring the sugars released from the insoluble cell wall matrix resulting from the action of bound enzymes. Autolytic activities were observed in the cell walls of elongating young leaves, mesocotyl and roots of maize. Wall proteins extracted from all of these structures are enriched in several types of glucanases and other wall polysaccharide hydrolases. These enzymes therefore appear to have a widespread and fundamental role in wall metabolism in growing tissues.
外切葡聚糖酶和内切葡聚糖酶介导细胞壁（1,3）（1,4）-β-D-葡聚糖的特异性降解，并且这些酶与生长素介导的谷物胚芽鞘生长和发育有关。然而，它们在其他组织中的分布和功能尚未完全明确。在本研究中，测定了不同玉米器官的葡聚糖酶活性和细胞壁自溶活性。自溶分析通过测量由结合酶作用从不溶性细胞壁基质释放的糖来评估细胞壁聚合物的原位水解。在玉米伸长的幼叶、中胚轴和根的细胞壁中观察到自溶活性。从所有这些结构中提取的壁蛋白富含几种类型的葡聚糖酶和其他壁多糖水解酶。因此，这些酶似乎在生长组织的壁代谢中具有广泛而重要的作用。
