Harris A R
CSIRO Division of Soils, and Cooperative Research Centre for Soil and Land Management, Glen Osmond, SA, Australia.
Microbiol Res. 2000 Mar;154(4):333-7. doi: 10.1016/S0944-5013(00)80007-5.
Two isolates of binucleate Rhizoctonia spp., previously selected for efficacy in suppression of Rhizoctonia solani and Pythium spp., as well as plant growth promotion, were incorporated into various solid substrate formulations. These formulated products were assayed at three doses in three glass-house experiments for biocontrol of damping-off diseases in Capsicum annuum. R. solani anastomosis group 4 or Pythium ultimum var. sporangiiferum were incorporated into pasteurized potting medium with each formulated binucleate Rhizoctonia product. All formulations were effective against both pathogens in at least two experiments, but some formulations of one isolate of binucleate Rhizoctonia did not give consistent control of R. solani in one experiment. The most consistent formulation, which provided control of both pathogens at all doses of binucleate Rhizoctonia, was the simple substrate of rice hulls. The implications for commercialization of a biocontrol product are discussed.
两种双核丝核菌属分离株，之前因其在抑制茄丝核菌和腐霉菌以及促进植物生长方面的功效而被选出，被制成各种固体基质配方产品。这些配方产品在三个温室实验中以三种剂量进行了测定，用于防治辣椒猝倒病。将茄丝核菌融合群4或终极腐霉变种孢囊梗腐霉与每种双核丝核菌配方产品一起加入经过巴氏杀菌的盆栽基质中。所有配方产品在至少两个实验中对两种病原菌均有效，但一种双核丝核菌分离株的某些配方产品在一个实验中对茄丝核菌的防治效果不一致。最稳定的配方产品是稻壳这种简单基质，在双核丝核菌的所有剂量下都能对两种病原菌起到防治作用。文中还讨论了生物防治产品商业化的相关问题。
