cDNA微阵列的标准化策略。
Normalization strategies for cDNA microarrays.
作者信息
Schuchhardt J, Beule D, Malik A, Wolski E, Eickhoff H, Lehrach H, Herzel H
机构信息
Institute for Theoretical Biology, Humboldt-Universität zu Berlin, Invalidenstrasse 43, D-10115 Berlin, Germany.
出版信息
Nucleic Acids Res. 2000 May 15;28(10):E47. doi: 10.1093/nar/28.10.e47.
Abstract
Multiple Arabidopsis thaliana clones from an experimental series of cDNA microarrays are evaluated in order to identify essential sources of noise in the spotting and hybridization process. Theoretical and experimental strategies for an improved quantitative evaluation of cDNA microarrays are proposed and tested on a series of differently diluted control clones. Several sources of noise are identified from the data. Systematic and stochastic fluctuations in the spotting process are reduced by control spots and statistical techniques. The reliability of slide to slide comparison is critically assessed within the statistical framework of pattern matching and classification.
摘要
对来自一系列cDNA微阵列实验的多个拟南芥克隆进行评估，以确定点样和杂交过程中噪声的主要来源。提出了改进cDNA微阵列定量评估的理论和实验策略，并在一系列不同稀释度的对照克隆上进行了测试。从数据中识别出几个噪声源。通过对照点和统计技术减少点样过程中的系统波动和随机波动。在模式匹配和分类的统计框架内，严格评估了玻片间比较的可靠性。
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