Hyde L A, Sherman G F, Denenberg V H
Dyslexia Research Laboratory, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Brain Res. 2000 Apr 28;863(1-2):151-9. doi: 10.1016/s0006-8993(00)02113-2.
Recently, we published a method for examining working and reference memory in mice using a spatial version of the water radial-arm maze. Here we describe a non-spatial version of the same maze. BXSB mice were able to learn the maze as shown by the decrease in the number of working and reference memory errors over sessions. This maze was used to examine learning differences between males and females and between mice with misplaced clusters of neurons in layer I of cortex (ectopias) and those without. In a prior study using the spatial version of the water radial-arm maze, male BXSB mice had poorer working memory than females during the acquisition phase. Similarly, in this study male BXSB mice demonstrated impaired working memory during the asymptotic phase of non-spatial radial-arm maze learning. Two prior studies showed that mice with neocortical ectopias demonstrated working memory impairments compared to non-ectopic littermates in the spatial version of the water radial-arm maze. Contrary to this, in the non-spatial radial-arm maze used here, ectopic mice were not impaired in working memory and showed better memory when the working memory 'load' was the highest. Overall, both versions of the maze can be useful tools to assess spatial and non-spatial working and reference memory in mice.
最近，我们发表了一种利用水迷宫的空间版本来检测小鼠工作记忆和参考记忆的方法。在此，我们描述同一迷宫的非空间版本。BXSB小鼠能够学会该迷宫，这可从各实验阶段工作记忆和参考记忆错误数量的减少得以体现。此迷宫用于检测雄性和雌性小鼠之间以及皮层I层神经元簇位置异常（异位）的小鼠与无此情况的小鼠之间的学习差异。在先前一项使用水迷宫空间版本的研究中，雄性BXSB小鼠在习得阶段的工作记忆比雌性小鼠差。同样，在本研究中，雄性BXSB小鼠在非空间放射状臂迷宫学习的渐近阶段表现出工作记忆受损。之前的两项研究表明，在水迷宫空间版本中，与无新皮质异位的同窝小鼠相比，有新皮质异位的小鼠表现出工作记忆受损。与此相反，在此处使用的非空间放射状臂迷宫中，异位小鼠的工作记忆未受损，并且在工作记忆“负荷”最高时表现出更好的记忆。总体而言，两种版本的迷宫都是评估小鼠空间和非空间工作记忆及参考记忆的有用工具。
