Hopmans E C, Schouten S, Pancost R D, van der Meer M T, Sinninghe Damsté J S
Netherlands Institute for Sea Research, Department of Marine Biogeochemistry and Toxicology, Texel.
Rapid Commun Mass Spectrom. 2000;14(7):585-9. doi: 10.1002/(SICI)1097-0231(20000415)14:7<585::AID-RCM913>3.0.CO;2-N.
A method combining normal phase high performance liquid chromatography (HPLC) with positive ion atmospheric pressure chemical ionization mass spectrometry (APCI-MS) was developed for the analysis of intact glycerol dialkyl glycerol tetraethers (GDGTs) in archaeal cell material and sediments. All GDGTs previously reported to occur in the thermophilic archaeon Sulfolobus solfataricus could be identified based on their mass spectra and retention time. Positive ion mass spectra consisted of abundant protonated molecules and fragment ions corresponding to loss of water and the glycerol moiety. In addition, two novel GDGTs representing alternative combinations of biphytanyl moieties were observed. Using this method, the tetraethers present in the thermophilic archaeon Metallosphaera sedula and two sediment samples were characterized. This rapid method will greatly contribute to the establishment of the sedimentary record of these compounds and increase our understanding of archaea and their occurrence in widely different environments.
开发了一种将正相高效液相色谱（HPLC）与正离子大气压化学电离质谱（APCI-MS）相结合的方法，用于分析古菌细胞材料和沉积物中的完整甘油二烷基甘油四醚（GDGTs）。根据先前报道的嗜热古菌嗜热栖热菌中存在的所有GDGTs的质谱和保留时间，可对其进行鉴定。正离子质谱由丰富的质子化分子和对应于失水和甘油部分损失的碎片离子组成。此外，还观察到了两种代表双植烷基部分替代组合的新型GDGTs。使用该方法，对嗜热古菌嗜热栖热菌和两个沉积物样品中存在的四醚进行了表征。这种快速方法将极大地有助于建立这些化合物的沉积记录，并增进我们对古菌及其在广泛不同环境中出现情况的理解。
