Department of Chemistry, University of York, York, YO10 5DD, UK.
Chem Phys Lipids. 2012 Sep;165(6):648-55. doi: 10.1016/j.chemphyslip.2012.06.009. Epub 2012 Jul 7.
Liquid chromatography-tandem mass spectrometry of membrane lipid cores from Sulfolobus species reveals isomeric forms of ring-containing isoprenoid glycerol dialkyl glycerol tetraether components not previously recognised via the use of NMR and liquid chromatography-mass spectrometry techniques. Equivalent isomerism was confirmed for the components in other hyperthermophilic genera and in sediments which contain the lipids of mesophilic archaea. The recognition of the isomeric structures in distinct archaeal clades suggests that profiles of tetraether lipids reported previously may have oversimplified the true lipid complexity in archaeal cultures and natural environments. Accordingly, the extent of variation in tetraether structures revealed by the work should direct more informative interpretations of lipid profiles in the future. Moreover, the results emphasise that tandem mass spectrometry provides a unique capability for assigning the structures of intact tetraether lipid cores for co-eluting species during chromatographic separation.
从 Sulfolobus 物种的膜脂核心的液相色谱-串联质谱分析中揭示了以前通过 NMR 和液相色谱-质谱技术无法识别的含环类异戊二烯甘油二烷基甘油四醚成分的同分异构体形式。在其他高温微生物属和含有中温古菌脂质的沉积物中，证实了这些成分的等同异构性。在不同古菌分支中识别出的异构结构表明，以前报道的四醚脂质图谱可能使古菌培养物和自然环境中真实的脂质复杂性过于简单化。因此，这项工作揭示的四醚结构的变化程度，应该可以为今后对脂质图谱进行更具信息量的解释提供指导。此外，研究结果强调了串联质谱在色谱分离过程中为共洗脱物种分配完整四醚脂质核心结构方面提供了独特的能力。
