Bray J E, Todd A E, Pearl F M, Thornton J M, Orengo C A
Biomolecular Structure and Modelling Unit, Department of Biochemistry and Molecular Biology, University College London, Gower Street,London WC1E 6BT, UK.
Protein Eng. 2000 Mar;13(3):153-65. doi: 10.1093/protein/13.3.153.
A consensus approach has been developed for identifying distant structural homologues. This is based on the CATH Dictionary of Homologous Superfamilies (DHS), a database of validated multiple structural alignments annotated with consensus functional information for evolutionary protein superfamilies (URL: http://www. biochem.ucl.ac.uk/bsm/dhs). Multiple structural alignments have been generated for 362 well-populated superfamilies in the CATH structural domain database and annotated with secondary structure, physicochemical properties, functional sequence patterns and protein-ligand interaction data. Consensus functional information for each superfamily includes descriptions and keywords extracted from SWISS-PROT and the ENZYME database. The Dictionary provides a powerful resource to validate, examine and visualize key structural and functional features of each homologous superfamily. The value of the DHS, for assessing functional variability and identifying distant evolutionary relationships, is illustrated using the pyridoxal-5'-phosphate (PLP) binding aspartate aminotransferase superfamily. The DHS also provides a tool for examining sequence-structure relationships for proteins within each fold group.
已经开发出一种用于识别远距离结构同源物的共识方法。这是基于同源超家族CATH词典（DHS），它是一个经过验证的多重结构比对数据库，注释了进化蛋白超家族的共识功能信息（网址：http://www.biochem.ucl.ac.uk/bsm/dhs）。已为CATH结构域数据库中362个丰富的超家族生成了多重结构比对，并注释了二级结构、物理化学性质、功能序列模式和蛋白质-配体相互作用数据。每个超家族的共识功能信息包括从SWISS-PROT和ENZYME数据库中提取的描述和关键词。该词典为验证、检查和可视化每个同源超家族的关键结构和功能特征提供了强大的资源。使用吡哆醛-5'-磷酸（PLP）结合天冬氨酸转氨酶超家族说明了DHS在评估功能变异性和识别远距离进化关系方面的价值。DHS还提供了一个工具，用于检查每个折叠组内蛋白质的序列-结构关系。
