Jaynes C D, Turner J E
Biology Department, Daemen College, Amherst, NY 14226, USA.
Brain Res Dev Brain Res. 2000 Apr 14;120(2):267-71. doi: 10.1016/s0165-3806(00)00017-1.
Retinal pigment epithelial cells (RPE) secrete a factor(s) in vitro which promotes Müller cell proliferation. To begin the isolation of the active factor, medium conditioned by cultured RPE (RPE-CM) was fractionated by fast protein liquid chromatography. The fractions were tested in an assay for Müller cell proliferation and it was found that one fraction contained biological activity comparable to that of complete RPE-CM. Gel electrophoresis demonstrated that this fraction consisted of proteins with approximate molecular masses of 35-88 kDa. An affinity assay revealed that only an 88-kDa protein in the RPE-CM binds to Müller cells. This protein was also present in the active fraction; therefore, it is the most likely source of the mitogenic activity of RPE-CM.
视网膜色素上皮细胞（RPE）在体外分泌一种促进 Müller 细胞增殖的因子。为了开始分离活性因子，通过快速蛋白质液相色谱法对培养的 RPE 条件培养基（RPE-CM）进行分级分离。在 Müller 细胞增殖测定中对这些级分进行了测试，发现其中一个级分具有与完整 RPE-CM 相当的生物活性。凝胶电泳表明该级分由分子量约为 35 - 88 kDa 的蛋白质组成。亲和测定显示 RPE-CM 中只有一种 88-kDa 的蛋白质与 Müller 细胞结合。这种蛋白质也存在于活性级分中；因此，它最有可能是 RPE-CM 促有丝分裂活性的来源。
