Szymanski M, Barciszewski J
Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Poznan, Poland.
IUBMB Life. 2000 Feb;49(2):121-3. doi: 10.1080/15216540050022430.
This paper describes our finding on overlapping genes in Methanococcus jannaschii genome. We found that one of the open reading frames (ORFs) within the M. jannaschii genome contains the nucleotide sequence of tRNA(Ser), which raises a serious question of the correctness of the initiation codon assignment for that ORF. We suggest that there are two other possible AUG initiation codons downstream from the TTG triplet, which was initially considered as a translation start site. Only one of the AUG triplets is preceded by the Shine-Dalgarno sequence that seems to be required for binding the ribosome and initiation of translation.
本文描述了我们对詹氏甲烷球菌基因组中重叠基因的研究发现。我们发现，詹氏甲烷球菌基因组中的一个开放阅读框（ORF）包含tRNA(Ser)的核苷酸序列，这就该ORF起始密码子分配的正确性提出了一个严峻的问题。我们认为，在最初被视为翻译起始位点的TTG三联体下游还有另外两个可能的AUG起始密码子。其中只有一个AUG三联体之前有Shine-Dalgarno序列，而该序列似乎是核糖体结合和翻译起始所必需的。
