Pichan D, Thomas M L, Damodaran A, Fakih A R, Chiplunkar S V
Cellular Immunology Unit, Cancer Research Institute, Tata Memorial Centre, Mumbai, India.
Tumori. 2000 Jan-Feb;86(1):64-9. doi: 10.1177/030089160008600112.
Tumor associated antigen (TAA) on oral squamous cell carcinoma (SCC) was characterized using the monoclonal antibody (MAb) 3F8E3. Flow cytometric analysis revealed a varying degree of reactivity of MAb 3F8E3 to TAA on oral tumor cells. Pretreatment of SCC cells with pronase and trypsin annulled the reactivity of MAb 3F8E3. Sodium metaperiodate (NaIO4) and neuraminidase marginally enhanced the binding of 3F8E3 on oral SCC cells. The studies indicate that the TAA recognized by MAb 3F8E3 on oral tumors is a protein moiety. On Western blotting MAb 3F8E3 showed reactivity to proteins with a molecular weight of 60-66 kDa on oral tumor lysates. MAb 3F8E3 reacted strongly to recombinant human hsp60 and 70 in ELISA. The results suggest that MAb 3F8E3 may react to an epitope expressed on a family of heat shock proteins.
使用单克隆抗体（MAb）3F8E3对口腔鳞状细胞癌（SCC）上的肿瘤相关抗原（TAA）进行了表征。流式细胞术分析显示MAb 3F8E3对口腔肿瘤细胞上的TAA具有不同程度的反应性。用链霉蛋白酶和胰蛋白酶预处理SCC细胞消除了MAb 3F8E3的反应性。偏高碘酸钠（NaIO4）和神经氨酸酶略微增强了3F8E3与口腔SCC细胞的结合。研究表明，MAb 3F8E3在口腔肿瘤上识别的TAA是一种蛋白质部分。在蛋白质印迹法中，MAb 3F8E3对口腔肿瘤裂解物上分子量为60 - 66 kDa的蛋白质有反应性。在酶联免疫吸附测定（ELISA）中，MAb 3F8E3对重组人热休克蛋白60和70有强烈反应。结果表明，MAb 3F8E3可能与热休克蛋白家族上表达的一个表位发生反应。
