Tatake R J, Amin K M, Maniar H S, Jambhekar N A, Srikhande S S, Gangal S G
Immunology Division, Tata Memorial Hospital, Parel, Bombay, India.
Int J Cancer. 1989 Nov 15;44(5):840-5. doi: 10.1002/ijc.2910440516.
Mouse monoclonal antibody (MAb) 3F8E3 (IgG3k) was developed against the head and neck cancer cell line LICR-LON-HN2. Subjected to indirect immunofluorescence, the MAb reacted exclusively with SCC cell lines and showed no reactivity with normal or transformed mouse and human non-SCC cell lines and hematopoietic cell lines. The radiolabelled MAb showed an affinity constant of 1.8 x 10(8) M-1 with HN2 cells and identified 2.07 x 10(4) sites/cell by Scatchard analysis. It identified 2 peptides from membrane extracts of HN2 cells by Western blotting. Avidin-biotin-complexed immunoperoxidase staining on cryostat sections of tumors from various tissues revealed that 3F8E3 reacted mainly with the membrane antigens of well differentiated SCC cells of oral cavity, larynx, esophagus, lung, uterine cervix, metastatic nodes of patients with oral cancer, and dysplastic cells in oral leukoplakia. The MAb did not react with poorly differentiated cells of Ca esophagus, adenocarcinoma of breast, stomach and colon, renal-cell carcinoma and soft-tissue sarcoma.
小鼠单克隆抗体（MAb）3F8E3（IgG3k）是针对头颈癌细胞系LICR-LON-HN2研制的。经间接免疫荧光检测，该单克隆抗体仅与鳞状细胞癌（SCC）细胞系发生反应，而与正常或转化的小鼠及人非SCC细胞系和造血细胞系无反应。放射性标记的单克隆抗体与HN2细胞的亲和常数为1.8×10⁸ M⁻¹，通过Scatchard分析确定每个细胞有2.07×10⁴个结合位点。通过蛋白质印迹法，它从HN2细胞膜提取物中鉴定出2种肽。对来自各种组织的肿瘤冷冻切片进行抗生物素蛋白-生物素复合物免疫过氧化物酶染色显示，3F8E3主要与口腔、喉、食管、肺、子宫颈的高分化SCC细胞、口腔癌患者的转移淋巴结以及口腔白斑中的发育异常细胞的膜抗原发生反应。该单克隆抗体与食管低分化细胞、乳腺腺癌、胃癌、结肠癌、肾细胞癌和软组织肉瘤无反应。
