可溶性鸟苷酸环化酶活性在豚鼠内耳中的定位。
Localization of soluble guanylate cyclase activity in the guinea pig inner ear.
作者信息
Takumida M, Anniko M, Popa R, Zhang D M
机构信息
Deportment of Otolaryngology, Hiroshima University School of Medicine, Japan.
出版信息
Acta Otolaryngol. 2000 Jan;120(1):28-33. doi: 10.1080/000164800760370792.
The aim of this study was to characterize the nitric oxide (NO) receptor soluble guanylate cyclase (sGC), to determine the cells targeted by NO and to elucidate the function of the NO/cGMP pathway in the inner ear. sGC activity in the inner ear was localized by immunohistochemical detection of NO-stimulated cGMP. Soluble guanylate cyclase activity in the cochlea was detected in the nerve endings underneath the outer and inner hair cells, supporting cells, stria vascularis and vessels. In the vestibular organs, sGC activity was detected in the cytoplasm of sensory cells, nerve fibres, dark cells and transitional cells and vessels. These findings suggest that the NO/cGMP pathway may be involved in regulatory processes in neurotransmission, blood flow and inner ear fluid homeostasis.
本研究的目的是对一氧化氮（NO）受体可溶性鸟苷酸环化酶（sGC）进行表征，确定NO作用的细胞，并阐明内耳中NO/cGMP信号通路的功能。通过免疫组化检测NO刺激产生的cGMP来定位内耳中的sGC活性。在耳蜗中，外毛细胞和内毛细胞下方的神经末梢、支持细胞、血管纹和血管中检测到可溶性鸟苷酸环化酶活性。在前庭器官中，在感觉细胞、神经纤维、暗细胞、过渡细胞和血管的细胞质中检测到sGC活性。这些发现表明，NO/cGMP信号通路可能参与神经传递、血流和内耳液体稳态的调节过程。
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