Myung N, Kim M R, Chung I P, Kim H, Jang J J
Department of Pathology, DanKook University, College of Medicine, Cheonan, South Korea.
Cancer Lett. 2000 May 29;153(1-2):129-36. doi: 10.1016/s0304-3835(00)00359-1.
We performed the immunohistochemical staining for six G1 check point cell cycle proteins to study their expression patterns and roles in the gastric carcinogenesis. We studied 76 cases of paraffin blocks that included the sections of 18 tubular adenomas (TA), 38 early gastric carcinomas (EGC) (20 cases of mucosal type, nine cases of submucosal type with no nodal metastasis, nine cases of submucosal type with nodal metastasis), 20 advanced gastric carcinomas (AGC) (ten cases with no nodal metastasis, ten cases with nodal metastasis). We found that abnormal expression of p16 and p27 increased with the progression of tubular adenomas to advanced gastric cancers. Inverse relationship between pRb and p16 proteins was found in a small portion of the gastric tumors. Expressions of pRb and cdk4 were consistently high in benign and malignant gastric tumors. Expression of cyclin D1 and cyclin E rather decreased with the tumor progression. In conclusion, losses of p16 and p27 seem to play a significant role during the gastric carcinogenesis, and the G1 checkpoint cell cycle proteins such as pRb, cdk4, cyclin D1, and cyclin E variably participate in the gastric carcinogenesis and metastasis by the mechanisms which are yet unknown; thus, further studies need to be performed to elucidate the mechanisms.
我们对六种G1期细胞周期检查点蛋白进行了免疫组化染色，以研究它们在胃癌发生过程中的表达模式和作用。我们研究了76例石蜡块，其中包括18例管状腺瘤（TA）、38例早期胃癌（EGC）（20例黏膜型、9例无淋巴结转移的黏膜下型、9例有淋巴结转移的黏膜下型）、20例进展期胃癌（AGC）（10例无淋巴结转移、10例有淋巴结转移）的切片。我们发现，随着管状腺瘤进展为进展期胃癌，p16和p27的异常表达增加。在一小部分胃肿瘤中发现了pRb和p16蛋白之间的负相关关系。pRb和cdk4在良性和恶性胃肿瘤中的表达始终较高。细胞周期蛋白D1和细胞周期蛋白E的表达反而随着肿瘤进展而降低。总之，p16和p27的缺失似乎在胃癌发生过程中起重要作用，而pRb、cdk4、细胞周期蛋白D1和细胞周期蛋白E等G1期细胞周期检查点蛋白通过尚不清楚的机制不同程度地参与胃癌的发生和转移；因此，需要进一步研究以阐明其机制。
