Mizunoe Y, Wai S N, Ishikawa T, Takade A, Yoshida S
Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
FEMS Microbiol Lett. 2000 May 1;186(1):115-20. doi: 10.1111/j.1574-6968.2000.tb09091.x.
Vibrio parahaemolyticus is known to exist in a viable but nonculturable state when incubated at low temperature under starvation. It has long been debated whether the culturable cells which appear after temperature upshift are the result of true resuscitation or regrowth of a few residual culturable cells. Starved V. parahaemolyticus cells at 4 degrees C reached the nonculturable stage in about 12 days. The true resuscitation of nonculturable cells of V. parahaemolyticus occurred after spreading them onto an agar medium supplemented with H(2)O(2)-degrading compounds such as catalase or sodium pyruvate. The proposed method may be applicable to detecting the enteropathogen from environmental samples.
已知副溶血性弧菌在低温饥饿培养时会进入活的非可培养状态。温度升高后出现的可培养细胞是真正复苏的结果还是少数残留可培养细胞生长的结果，长期以来一直存在争议。在4摄氏度下饥饿培养的副溶血性弧菌细胞大约在12天后进入非可培养阶段。将副溶血性弧菌的非可培养细胞铺在添加了过氧化氢降解化合物（如过氧化氢酶或丙酮酸钠）的琼脂培养基上后，会发生真正的复苏。所提出的方法可能适用于从环境样本中检测肠道病原体。
