Furman D P, Omel'ianchuk L V
Institute of Cytology and Genetics, Russian Academy of Science, Novosibirsk, Russia.
Genetika. 2000 Mar;36(3):348-56.
In this work, critical ontogenetic stages for wing traits affected by temperature-sensitive mutation Walker (Wk) were determined. The interaction between the Wk gene and some genes responsible for the cell-cycle control was studied. At various ontogenetic stages, the mutants were exposed to 17 degrees C for 12 h, and, at the beginning of oviposition, the fly age was registered. Nine types of wing abnormalities were classified. The temperature treatment during three developmental stages (12-24, 48-60, and 96-108 h) resulted in a decrease in normal wing number and a substantial increase in wing abnormalities. Different morphological types of imaginal disks were revealed: nondifferentiated disks, those lacking the notum region, and those with duplications of wing-forming regions. The allele-specific interaction between Wk and allele v27 of the Klp61F gene was also revealed. We suggest that gene Wk is a high-ranking gene in the system of genetic control of ontogeny, because the Wk mutation is manifested in numerous phenotypic variants both in the control and in the experiment and a complete set of these variants was observed at each developmental stage upon temperature treatment. The pleiotropic effect of the Wk gene on the formation of some Drosophila organs, including eyes and halters which are beyond the scope of this report, is in agreement with this suggestion.
在本研究中，确定了受温度敏感突变体沃克（Wk）影响的翅性状的关键个体发育阶段。研究了Wk基因与一些负责细胞周期调控的基因之间的相互作用。在不同的个体发育阶段，将突变体置于17摄氏度环境中12小时，并在产卵开始时记录果蝇的年龄。对九种翅异常类型进行了分类。在三个发育阶段（12 - 24小时、48 - 60小时和96 - 108小时）进行温度处理，导致正常翅数量减少，翅异常显著增加。揭示了不同形态类型的成虫盘：未分化的盘、缺少背板区域的盘以及翅形成区域重复的盘。还揭示了Wk与Klp61F基因的v27等位基因之间的等位基因特异性相互作用。我们认为，基因Wk在个体发育的遗传控制系统中是一个高级基因，因为Wk突变在对照和实验中均表现为众多表型变异，并且在温度处理后的每个发育阶段都观察到了这些变异的完整集合。Wk基因对包括眼睛和平衡棒（本报告未涉及）在内的一些果蝇器官形成的多效性效应与这一观点相符。
