Chow A W, Patten V, Marshall J R
Am J Obstet Gynecol. 1979 Feb 15;133(4):362-5. doi: 10.1016/0002-9378(79)90051-6.
In our earlier study, Neisseria gonorrhoeae was infrequently isolated while Bacteroides fragilis was totally absent from cul-de-sac aspirates of patients with acute pelvic inflammatory disease (PID). Twenty additional women were studied to further elucidate the bacteriology of this disease. Recovery rate of N. gonorrhoeae from cul-de-sac aspirates collected in selective transport medium (Transgrow) was 25 per cent among patients with gonococcal PID, compared to only 8 per cent when specimens were collected in a nonselective transport system (Anaport). In vitro studies confirmed a detrimental effect of the Anaport for survival of N. gonorrhoeae during transport. This suboptimal survival was primarily related to low pH of the transport system under prereduced conditions, and could be corrected when pH of the transport vial was preadjusted and maintained between 6 and 7. B. fragilis was again absent from the cul-de-sac in all patients, while streptococci, peptococci, and peptostreptococci remained the most predominant isolates. These data may explain the empiric observations that penicillin and tetracycline are effective in most cases of acute PID.
在我们早期的研究中，淋病奈瑟菌很少分离得到，而急性盆腔炎（PID）患者的阴道后穹窿吸出物中完全没有脆弱拟杆菌。另外对20名女性进行了研究，以进一步阐明该疾病的细菌学情况。在患有淋菌性PID的患者中，从收集于选择性转运培养基（Transgrow）中的阴道后穹窿吸出物中分离出淋病奈瑟菌的回收率为25%，而当标本收集于非选择性转运系统（Anaport）中时，回收率仅为8%。体外研究证实，Anaport对淋病奈瑟菌在转运过程中的存活有不利影响。这种存活不佳主要与预还原条件下转运系统的低pH值有关，当转运瓶的pH值预先调整并维持在6至7之间时，这种情况可以得到纠正。所有患者的阴道后穹窿中再次未发现脆弱拟杆菌，而链球菌、消化球菌和消化链球菌仍然是最主要的分离菌株。这些数据可能解释了青霉素和四环素在大多数急性PID病例中有效的经验性观察结果。
