Suzuki T, Yamada M, Ishida T, Morii T, Makino K
Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Japan.
Nucleic Acids Symp Ser. 1999(42):7-8. doi: 10.1093/nass/42.1.7.
2'-Deoxyoxanosine (dOxo) is a novel DNA lesion produced from 2'-deoxyguanosine by the reaction with nitrous acid or nitric oxide. We found that dOxo reacted with glycine under physiological conditions. The product was identified by spectrometric data as an adduct between the six membered ring of dOxo and an amino group of glycine. The adduct was more stable than dOxo under physiological conditions. The incubation of an oligodeoxynucleotide containing dOxo with glycine gave also rise to the adduct. These results suggest that dOxo formed in DNA reacts with amino groups of various compounds around DNA in vivo resulting in the adduct.
2'-脱氧氧杂鸟苷（dOxo）是由2'-脱氧鸟苷与亚硝酸或一氧化氮反应产生的一种新型DNA损伤。我们发现dOxo在生理条件下与甘氨酸发生反应。通过光谱数据鉴定该产物为dOxo的六元环与甘氨酸的氨基之间的加合物。在生理条件下，该加合物比dOxo更稳定。含有dOxo的寡脱氧核苷酸与甘氨酸一起温育也会产生该加合物。这些结果表明，在DNA中形成的dOxo在体内与DNA周围各种化合物的氨基发生反应，从而产生加合物。
