Yamada M, Honjo H, Hasegawa T, Morimoto E, Suzuki T, Morii T, Makino K
Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan.
Nucleic Acids Res Suppl. 2001(1):159-60. doi: 10.1093/nass/1.1.159.
2'-Deoxyoxanosine (dOxo) is a novel DNA lesion produced from 2'-deoxyguanosine by the treatment with nitrous acid and nitric oxide. However, there has been no direct evidence on the formation of dOxo in vivo so far. In order to detect dOxo in vivo, we have explored a sensitive detection method specific for dOxo by using a fluorescent labeling reagent and HPLC analysis. An efficient method to detect the dOxo formation in DNA is described.
2'-脱氧氧杂鸟苷（dOxo）是由亚硝酸和一氧化氮处理2'-脱氧鸟苷产生的一种新型DNA损伤。然而，迄今为止尚无dOxo在体内形成的直接证据。为了在体内检测dOxo，我们利用荧光标记试剂和高效液相色谱分析探索了一种针对dOxo的灵敏检测方法。本文描述了一种检测DNA中dOxo形成的有效方法。
