聚阳离子梳型共聚物对DNA链交换的加速作用。

Acceleration of DNA strand exchange by polycation comb-type copolymer.

作者信息

Kim W J, Ishihara T, Akaike T, Maruyama A

机构信息

Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

Nucleic Acids Symp Ser. 1999(42):139-40. doi: 10.1093/nass/42.1.139.

DOI:10.1093/nass/42.1.139

PMID:10780418

Abstract

Polycation comb-type copolymers which are composed of poly(L-lysine) backbone and dextran graft chain (PLL-graft-Dex) accelerated DNA duplex and triplex formation and stabilized under physiologically relevant condition remarkably. In this study, we have examined the ability of polycation copolymer in promoting strand exchange between duplex DNA and its complementary single-stranded DNA. It was demonstrated that the strand exchange rate was considerably accelerated by the polycation comb-type copolymer.

摘要

由聚（L-赖氨酸）主链和葡聚糖接枝链组成的聚阳离子梳型共聚物（PLL-接枝-Dex）显著加速了DNA双链体和三链体的形成，并在生理相关条件下具有显著的稳定性。在本研究中，我们检测了聚阳离子共聚物促进双链DNA与其互补单链DNA之间链交换的能力。结果表明，聚阳离子梳型共聚物显著加速了链交换速率。