用腺病毒介导的小鼠白细胞介素-4基因转移对实验性小鼠脑肿瘤进行免疫基因治疗。
Immune gene therapy of experimental mouse brain tumor with adenovirus-mediated gene transfer of murine interleukin-4.
作者信息
Yoshikawa K, Kajiwara K, Ideguchi M, Uchida T, Ito H
机构信息
Department of Neurosurgery, Yamaguchi University School of Medicine, Ube, Japan.
出版信息
Cancer Immunol Immunother. 2000 Apr;49(1):23-33. doi: 10.1007/s002620050023.
We examined the antitumor activity of replication-deficient adenoviral vectors carrying the murine interleukin-4 (IL-4) gene (AdCIL4) using a syngeneic brain tumor model in mice. Mice implanted with malignant astrocytoma cells infected with AdCIL4 survived significantly longer than those in the control groups. Immunocytochemical analysis of the tumors showed that AdCIL4 caused the strong up-regulation of MHC class II antigen expression by the tumor cells and macrophages, and consequent infiltration by CD8+ T lymphocytes. This study demonstrates the efficacy of IL-4 gene transfection mediated by adenoviral vectors for intracerebral tumor and characterizes the immunoreaction caused by AdCIL4.
我们使用小鼠同基因脑肿瘤模型，研究了携带小鼠白细胞介素-4（IL-4）基因的复制缺陷型腺病毒载体（AdCIL4）的抗肿瘤活性。植入感染AdCIL4的恶性星形细胞瘤细胞的小鼠存活时间明显长于对照组。对肿瘤的免疫细胞化学分析表明，AdCIL4导致肿瘤细胞和巨噬细胞强烈上调MHC II类抗原表达，并随后有CD8 + T淋巴细胞浸润。本研究证明了腺病毒载体介导的IL-4基因转染对脑内肿瘤的疗效，并对AdCIL4引起的免疫反应进行了表征。
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