Morioka Jun, Kajiwara Koji, Yoshikawa Koichi, Ideguchi Makoto, Uchida Tetsuya, Ohmoto Yoshinori, Suzuki Michiyasu
Department of Neurosurgery, Yamaguchi University School of Medicine, Japan.
J Neurooncol. 2002 Oct;60(1):13-23. doi: 10.1023/a:1020260822669.
The purpose of the present study was to determine if adenovirus-mediated transfection of a syngeneic mouse brain tumor with the gene encoding B7.1 enhances immunogenicity against tumor. Malignant astrocytoma cells were transfected with adenoviral vectors carrying the B7.1 gene (AdB7). Immunocytochemical analysis confirmed the expression of B7.1 in vitro and in vivo. To investigate the effects of B7.1 expression on tumorigenicity of the malignant astrocytoma, mice were implanted intracerebrally with B7.1-transfected glioma cells. There was no significant difference in proliferation between B7.1-transfected cells and controls in vitro. Nevertheless, mice implanted with B7. 1-transfected cells survived significantly longer than those in the control groups. Immunocytochemical analysis of the tumors showed that there was infiltration of a number of CD8+ T-cells and CD25+ activated T-cells in the brain implanted with B7.1-transfected glioma cells. The results showed the possibility that adenovirus-mediated B7.1 gene transfection to a brain tumor induced activation of CD8+ cytotoxic T-lymphocytes.
本研究的目的是确定腺病毒介导的将编码B7.1的基因转染至同基因小鼠脑肿瘤中是否能增强抗肿瘤免疫原性。用携带B7.1基因的腺病毒载体（AdB7）转染恶性星形细胞瘤细胞。免疫细胞化学分析证实了B7.1在体内外的表达。为了研究B7.1表达对恶性星形细胞瘤致瘤性的影响，将转染了B7.1的胶质瘤细胞脑内植入小鼠。在体外，B7.1转染细胞与对照细胞之间的增殖没有显著差异。然而，植入转染了B7.1细胞的小鼠存活时间明显长于对照组。对肿瘤的免疫细胞化学分析表明，在植入转染了B7.1的胶质瘤细胞的脑内有大量CD8 + T细胞和CD25 + 活化T细胞浸润。结果表明腺病毒介导的B7.1基因转染脑肿瘤有诱导CD8 + 细胞毒性T淋巴细胞活化的可能性。
