Sprott L R, Harris M D, Forrest D W, Young J, Zhang H M, Oyarzo J N, Bellin M E, Ax R L
Department of Animal Science, Texas A&M University, College Station 77843, USA.
J Anim Sci. 2000 Apr;78(4):795-8. doi: 10.2527/2000.784795x.
In this study, semen samples from 25 bulls that had passed a breeding soundness evaluation were analyzed for the presence or absence of a 31-kDa protein, known as fertility-associated antigen (FAA), on spermatozoal membranes. Eighteen bulls had FAA on sperm (FAA-positive) and seven were devoid of FAA on sperm (FAA-negative). A single ejaculate from each bull was extended and frozen with 25 to 30 x 10(6) sperm in .5-mL straws. Crossbred replacement heifers (n = 865) were estrus-synchronized and artificially inseminated either at timed AI or 12 h after they were detected in estrus. Mature cows (n = 285) were inseminated 12 h after they were detected in estrus during a 45-d AI period. Pregnancy rates (pooled) to first AI service for females (n = 764) inseminated with FAA-positive sperm were 65.6% and were 49.7% for females (n = 386) inseminated with FAA-negative sperm (P < .005). Among the estrus-synchronized replacement heifers, pregnancy rates to synchronized AI service for heifers (n = 550) inseminated with FAA-positive sperm were 62% and were 45.7% for heifers (n = 315) inseminated with FAA-negative sperm (P < .005). These data indicate that pregnancy rates to first AI service at spontaneous and synchronized estrus are higher when using semen from bulls with detectable FAA on spermatozoal membranes compared to semen from bulls devoid of FAA on membranes. Fertility-associated antigen is an important determinant for fertility potential of sperm from bulls to be used in AI breeding programs.
在本研究中，对25头通过繁殖健全性评估的公牛的精液样本进行分析，以检测精子膜上是否存在一种名为生育相关抗原（FAA）的31 kDa蛋白质。18头公牛的精子上有FAA（FAA阳性），7头公牛的精子上没有FAA（FAA阴性）。从每头公牛采集一次射精样本，将其稀释并冷冻于0.5 mL细管中，每管含有25至30×10⁶个精子。对865头杂交后备小母牛进行发情同步处理，并在定时人工授精时或发情被检测到后12小时进行人工授精。在45天的人工授精期内，对285头成年母牛在发情被检测到后12小时进行授精。用FAA阳性精子授精的雌性（n = 764）首次人工授精服务的合并妊娠率为65.6%，用FAA阴性精子授精的雌性（n = 386）为49.7%（P < 0.005）。在发情同步的后备小母牛中，用FAA阳性精子授精的小母牛（n = 550）同步人工授精服务的妊娠率为62%，用FAA阴性精子授精的小母牛（n = 315）为45.7%（P < 0.005）。这些数据表明，与精子膜上没有FAA的公牛精液相比，使用精子膜上可检测到FAA的公牛精液时，自发发情和同步发情时首次人工授精服务的妊娠率更高。生育相关抗原是用于人工授精繁殖计划的公牛精子生育潜力的重要决定因素。
