Department of Animal and Veterinary Science, University of Idaho, Caldwell, Idaho, USA.
Theriogenology. 2012 Jan 15;77(2):389-94. doi: 10.1016/j.theriogenology.2011.08.011. Epub 2011 Sep 29.
The objective was to determine whether the presence of fertility-associated antigen (FAA) on sperm collected from Nelore (Bos indicus) bulls can be used to assess potential fertility of sperm for use at first-service fixed-time AI (TAI). Six Nelore bulls were selected based on FAA status (FAA-negative: N = 3; FAA-positive: N = 3) and the ability to produce neat semen with ≥ 70% morphologically normal sperm and 60% estimated progressive motility before cryopreservation. In Experiment 1, suckled multiparous Nelore cows (N = 835) were evaluated for body condition score (BCS) and received an intravaginal progesterone device (CIDR) and 2.0 mg of estradiol benzoate (Day 0). On Day 9 the CIDR was removed, 12.5 mg of PGF(2α) and 0.5 mg of estradiol cypionate were administered, and calves were removed for 48 h. All cows received TAI on Day 11 (48 h after CIDR removal). Pregnancy per TAI (P/TAI) was not different between FAA-positive and FAA-negative bulls (41.5% vs. 39.3%, respectively). There was an effect of AI technician on P/TAI (36.0% vs. 43.9%; P < 0.05) and BCS tended to affect P/TAI (P = 0.09), as cows with BCS ≥ 2.75 were 1.4 times more likely to become pregnant compared with cows with BCS < 2.75. In Experiment 2, nulliparous Nelore heifers (N = 617) were evaluated for BCS and received a CIDR and estradiol benzoate (2.0 mg) on Day 0. On Day 7, all heifers received PGF(2α) (12.5 mg). On Day 9, CIDR inserts were removed and all heifers received estradiol cypionate (0.6 mg) and 200 IU eCG. All heifers received TAI on Day 11 (48 h after CIDR removal). Pregnancy/TAI was different (P = 0.04) between FAA-positive and FAA-negative bulls (33.7% vs. 40.7%, respectively). Presence of FAA on sperm was unsuccessful in assessing the potential fertility of sperm for use in TAI.
目的是确定从内罗尔（Bos indicus）公牛中收集的精子是否存在生育相关抗原（FAA），是否可以用于评估用于首次定时人工授精（TAI）的精子的潜在生育能力。根据 FAA 状态（FAA 阴性：N = 3；FAA 阳性：N = 3）和冷冻保存前产生干净精液的能力，选择了 6 头内罗尔公牛，该精液具有≥70％形态正常的精子和≥60％的估计前向运动性。在实验 1 中，对哺乳期经产的内罗尔奶牛（N = 835）进行了体况评分（BCS）评估，并使用阴道内孕酮装置（CIDR）和 2.0 mg 苯甲酸雌二醇（第 0 天）。第 9 天取出 CIDR，给予 12.5mgPGF（2α）和 0.5mg 雌二醇环戊丙酸酯，并取出小牛 48 小时。所有母牛均于第 11 天（CIDR 取出后 48 小时）接受 TAI。FAA 阳性和 FAA 阴性公牛之间的每 TAI 妊娠率（P/TAI）没有差异（分别为 41.5％和 39.3％）。AI 技术员对 P/TAI 有影响（36.0％对 43.9％；P <0.05），BCS 也倾向于影响 P/TAI（P = 0.09），因为 BCS≥2.75 的母牛比 BCS <2.75 的母牛怀孕的可能性高 1.4 倍。在实验 2 中，对内罗尔小母牛（N = 617）进行了 BCS 评估，并在第 0 天使用 CIDR 和苯甲酸雌二醇（2.0mg）。第 7 天，所有小母牛均接受 PGF（2α）（12.5mg）。第 9 天，取出 CIDR 塞，并给所有小母牛注射雌二醇环戊丙酸酯（0.6mg）和 200IU eCG。所有小母牛均于第 11 天（CIDR 取出后 48 小时）接受 TAI。FAA 阳性和 FAA 阴性公牛之间的妊娠/TAI 不同（P = 0.04）（分别为 33.7％和 40.7％）。精子上 FAA 的存在无法评估用于 TAI 的精子的潜在生育能力。
