McNeel R L, Ding S T, Smith E O, Mersmann H J
USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030-2600, USA.
J Anim Sci. 2000 Apr;78(4):934-42. doi: 10.2527/2000.784934x.
To determine possible genetic influences on the steady-state concentrations of several key transcription factor transcripts and the transcript concentrations for adipocyte-characteristic proteins, young, genetically obese and lean pigs were given ad libitum access or feed or were restrictively fed at 50% of ad libitum intake for 5 wk. Obese pigs were smaller and fatter than lean pigs, whether intake was ad libitum or restrictive. Plasma protein, albumin, and cholesterol concentrations were greater in obese than in lean pigs. Plasma NEFA, blood urea nitrogen, triacylglycerols, and postprandial glucose and insulin concentrations were less (P < .02) in pigs fed restrictively than in pigs with ad libitum access to feed, regardless of genetic group. The adipose tissue glucose transporter 4, fatty acid synthase, and leptin transcript concentrations were greater (P < .05) in obese than in lean pigs. The CCAAT/enhancer binding proteins beta and alpha, adipocyte fatty acid binding protein, hormone-sensitive lipase, and the beta1-adrenergic receptor transcript concentrations tended (P < . 10) to be greater in adipose tissue from obese than in that from lean pigs. Several other transcripts were numerically greater in obese than in lean pigs. The data collectively suggest that messenger RNA concentration for several adipose tissue proteins is a contributing factor to the excess fat deposition in these obese pigs. Restricted feeding did not change the concentration of any transcript except that for adipocyte fatty acid binding protein, which was reduced. The accretion of fat was markedly reduced in the restrictively fed pigs, but this diminution does not seem to be regulated by modulation of messenger RNA concentration.
为了确定基因对几种关键转录因子转录本的稳态浓度以及脂肪细胞特征蛋白转录本浓度的可能影响，对幼年、遗传性肥胖和瘦猪进行自由采食或限食，限食为自由采食量的50%，持续5周。无论采食是自由采食还是限食，肥胖猪都比瘦猪体型小且更胖。肥胖猪的血浆蛋白、白蛋白和胆固醇浓度高于瘦猪。限食组猪的血浆非酯化脂肪酸、血尿素氮、三酰甘油以及餐后葡萄糖和胰岛素浓度低于自由采食组猪（P<0.02），无论基因分组如何。肥胖猪脂肪组织中的葡萄糖转运蛋白4、脂肪酸合酶和瘦素转录本浓度高于瘦猪（P<0.05）。肥胖猪脂肪组织中的CCAAT/增强子结合蛋白β和α、脂肪细胞脂肪酸结合蛋白、激素敏感性脂肪酶以及β1-肾上腺素能受体转录本浓度倾向于高于瘦猪（P<0.10）。肥胖猪中其他几种转录本的数值也高于瘦猪。这些数据共同表明，几种脂肪组织蛋白的信使核糖核酸浓度是这些肥胖猪脂肪过度沉积的一个促成因素。限食除了使脂肪细胞脂肪酸结合蛋白的转录本浓度降低外，没有改变任何转录本的浓度。限食组猪的脂肪蓄积明显减少，但这种减少似乎不是通过调节信使核糖核酸浓度来调控的。
