Spurlock M E, Frank G R, Cornelius S G, Ji S, Willis G M, Bidwell C A
Swine Research Group, Purina Mills, St. Louis, MO 63144, USA.
J Nutr. 1998 Apr;128(4):677-82. doi: 10.1093/jn/128.4.677.
The relationship between obese gene expression and energy intake was determined in pigs of various body weights. With ad libitum consumption, expression increased (P < 0.001) with body weight from 55 to 163 kg. Obese mRNA relative abundance was correlated with fat mass (r = 0.74, P < 0.0001) and percentage of fat (r = 0.72, P < 0. 0001). Obese expression was also evaluated at 159 kg (initial weight) and ad libitum, maintenance or 23% of maintenance intake for 28 d. Obese mRNA was independent of treatment (P > 0.78) despite considerable weight differences. Obese mRNA abundance was then compared at 136 kg (initial weight) and ad libitum or maintenance intake for 3 or 28 d. Abundance was not influenced by either duration of treatment or intake, despite a small increase (P < 0.01) in serum nonesterified fatty acids (NEFA) and a reduction (P < 0.02) in insulin attributable to maintenance intake. Finally, mRNA abundance was determined at 60 and 136 kg and conditions of food deprivation or ad libitum intake for 3 d. Food deprivation reduced (P < 0.01) serum insulin and increased (4- to 5-fold) NEFA concentrations. Obese mRNA abundance was greater (P < 0.01) in the heavier pigs and was reduced (P < 0.01) by food deprivation. We conclude that obese mRNA abundance in pigs correlates with fat mass and percentage of body fat under conditions of ad libitum intake. Furthermore, obese mRNA abundance is reduced by food deprivation, whereas lesser degrees of intake restriction do not change obese mRNA abundance, even when accompanied by appreciable weight loss.
在不同体重的猪中测定了肥胖基因表达与能量摄入之间的关系。自由采食时，随着体重从55千克增加到163千克，肥胖基因表达增加（P<0.001）。肥胖mRNA相对丰度与脂肪量（r = 0.74，P<0.0001）和脂肪百分比（r = 0.72，P<0.0001）相关。还在体重159千克（初始体重）时对肥胖基因表达进行了评估，自由采食、维持摄入量或维持摄入量的23%持续28天。尽管体重差异显著，但肥胖mRNA不受处理方式影响（P>0.78）。然后在体重136千克（初始体重）时比较了自由采食或维持摄入量3天或28天的肥胖mRNA丰度。尽管维持摄入量导致血清非酯化脂肪酸（NEFA）略有增加（P<0.01）和胰岛素降低（P<0.02），但肥胖mRNA丰度不受处理持续时间或摄入量影响。最后，在体重60千克和136千克时，测定了禁食或自由采食3天条件下的mRNA丰度。禁食降低了血清胰岛素水平（P<0.01），并使NEFA浓度增加了4至5倍。较重猪的肥胖mRNA丰度更高（P<0.01），禁食使其降低（P<0.01）。我们得出结论，在自由采食条件下，猪的肥胖mRNA丰度与脂肪量和体脂百分比相关。此外，禁食可降低肥胖mRNA丰度，而程度较轻的摄入量限制即使伴有明显体重减轻，也不会改变肥胖mRNA丰度。
