Smith A E, Harmon J P, Deogracias F A, Pizer E S
Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA.
Gynecol Oncol. 2000 May;77(2):336-8. doi: 10.1006/gyno.2000.5753.
OBJECTIVE(S): Most conditions involving sex cord-stromal cells can be diagnosed on morphologic criteria alone. We describe a case of vascular embolization of benign granulosa cells in which immunohistochemistry was of value as a diagnostic tool.
We reviewed the clinical history and gross pathologic findings from a 48-year-old patient who presented with abdominal pain and fullness. Formalin-fixed paraffin-embedded sections were examined by routine H&E and immunohistochemical stains.
Histologic examination of a grossly enlarged and cystic ovary revealed nests of cells within angiolymphatic spaces. Although the cells were cohesive and atypical, they were morphologically similar to the nearby graafian follicle. Immunohistochemistry showed positive labeling with antibodies to inhibin-alpha and cytokeratin in a pattern consistent with benign granulosa cells.
CONCLUSION(S): Immunohistochemical stains for inhibin-alpha and cytokeratin are useful tools to help confirm granulosa cell origin, as demonstrated in this case involving an atypical histomorphologic picture of "embolization."
大多数涉及性索间质细胞的疾病仅通过形态学标准即可诊断。我们描述了一例良性颗粒细胞血管栓塞病例，其中免疫组化作为一种诊断工具具有重要价值。
我们回顾了一名48岁出现腹痛和腹胀患者的临床病史及大体病理结果。对福尔马林固定石蜡包埋切片进行常规苏木精-伊红染色及免疫组化染色检查。
对一个明显肿大且呈囊性的卵巢进行组织学检查，发现血管淋巴管间隙内有细胞巢。尽管这些细胞黏附性强且形态不典型，但在形态上与附近的格拉夫卵泡相似。免疫组化显示，抗抑制素-α抗体和细胞角蛋白呈阳性标记，其模式与良性颗粒细胞一致。
如本病例中这种呈现“栓塞”非典型组织形态学表现所示，抑制素-α和细胞角蛋白的免疫组化染色是有助于确认颗粒细胞来源的有用工具。
