Tonder O, Humphrey L J, Morse P A
Cancer. 1975 Mar;35(3):580-7. doi: 10.1002/1097-0142(197503)35:3<580::aid-cncr2820350304>3.0.co;2-d.
Twenty human malignant solid tumors of various histologic types were tested for the presence of Fc receptor using cryostat sections or single cell suspensions of fresh tissue. Sheep erythrocytes sensitized by various amounts of rabbit IgG antibodies served as indicator cells (EA). All tumors possessed Fc receptor, but to varying degrees; eight reacted more strongly than normal spleen without any relation to histologic type. The tumors which gave the strongest reactions in sections also formed the highest percent of EA rosettes in suspensions, thus indicating surface localization of receptors. The reactions with spleen sections localized to the B cell and monocytic areas; the latter also showed high avidity in reactions with uncomplexed IgG. Rabbit antisera to tumors, spleen, and peripheral lymphocytes (polyvalent ALS) in inhibited the reactions, while a T-cell-specific ALS did not. Absorptions of the antisera with lymphocytes or tissue sediments of spleen and tumors removed the inhibiting activity, tissue sediments of muscle and kidney only reduced the titers. Again, results with spleen sections paralleled those obtained with tumor sections. Apparently, the tumor Fc receptor is very similar to the Fc receptors present in normal lymphoreticular tissues.
利用新鲜组织的冰冻切片或单细胞悬液，对20例不同组织学类型的人类恶性实体瘤进行了Fc受体检测。用不同量兔IgG抗体致敏的绵羊红细胞作为指示细胞（EA）。所有肿瘤均具有Fc受体，但程度不同；8例肿瘤的反应比正常脾脏更强，且与组织学类型无关。在切片中反应最强的肿瘤在悬液中形成EA花环的百分比也最高，这表明受体位于细胞表面。与脾脏切片的反应定位于B细胞和单核细胞区；后者在与未结合IgG的反应中也表现出高亲和力。兔抗肿瘤、脾脏和外周淋巴细胞抗血清（多价抗淋巴细胞血清）可抑制反应，而T细胞特异性抗淋巴细胞血清则不能。用淋巴细胞或脾脏及肿瘤的组织沉淀物吸收抗血清可去除抑制活性，肌肉和肾脏的组织沉淀物仅降低效价。同样，脾脏切片的结果与肿瘤切片的结果相似。显然，肿瘤Fc受体与正常淋巴网状组织中存在的Fc受体非常相似。
