兔心肌间质内肾上腺素在体内的局部释放

Local epinephrine release in the rabbit myocardial interstitium in vivo.

作者信息

Kawada T, Yamazaki T, Akiyama T, Sato T, Shishido T, Yoshimura R, Inagaki M, Tatewaki T, Sugimachi M, Sunagawa K

机构信息

Department of Cardiovascular Dynamics, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.

出版信息

J Auton Nerv Syst. 2000 Jan 14;78(2-3):94-8. doi: 10.1016/s0165-1838(99)00064-8.

DOI:10.1016/s0165-1838(99)00064-8

PMID:10789687

Abstract

Although several investigations have suggested cardiac epinephrine (Epi) release, local Epi release in the myocardial interstitium in vivo has not been measured. Using cardiac microdialysis in the rabbit, we measured dialysate Epi and norepinephrine (NE) concentrations as indices of myocardial interstitial Epi and NE levels, respectively. Exocytotic release induced by local administration of KCl (100 mM) through the dialysis probe increased Epi to 24.2 +/- 13.2 pg/ml from a control value of 3.2 +/- 3.6 pg/ml (P < 0.01, n = 6). Non-exocytotic release induced by the local administration of tyramine (10 microg/ml) also increased Epi to 34.6 +/- 15.3 pg/ml (p < 0.05 from control, n = 6). We conclude that Epi can be released via both exocytotic and non-exocytotic release mechanisms from the heart.

摘要

尽管多项研究表明心脏会释放肾上腺素（Epi），但尚未对体内心肌间质中局部Epi的释放进行测量。我们利用家兔心脏微透析技术，分别测定了透析液中Epi和去甲肾上腺素（NE）的浓度，以此作为心肌间质中Epi和NE水平的指标。通过透析探头局部注射氯化钾（100 mM）诱导的胞吐释放，使Epi从对照值3.2±3.6 pg/ml升高至24.2±13.2 pg/ml（P<0.01，n=6）。局部注射酪胺（10μg/ml）诱导的非胞吐释放也使Epi升高至34.6±15.3 pg/ml（与对照相比P<0.05，n=6）。我们得出结论，心脏中的Epi可通过胞吐和非胞吐释放机制释放。