Kalbitz K, Geyer S, Geyer W
Department of Soil Ecology, Bayreuth Institute for Terrestrial Ecosystem Research (BITOK), Germany.
Chemosphere. 2000 Jun;40(12):1305-12. doi: 10.1016/s0045-6535(99)00238-6.
The aim of our study is to test the use of less time-consuming spectroscopic methods applied on original water samples in order to obtain information about DOM composition without any sample preparation. These results were directly compared with results from a conventional isolation and characterization procedure of dissolved humic substances (fulvic acids--FA) isolated from the same water sample. FAs were characterized by UV-, fluorescence-, FTIR spectroscopy and elemental composition. UV absorbance and fluorescence behavior of FAs and original water samples follow the same pattern. A lower UV absorbance and a lower humification index (derived from the synchronous fluorescence spectra) of about 15% is typical for water samples compared to the FAs. We computed linear relationships between properties of the original water sample (UV-, synchronous fluorescence spectra) and the isolated FA (IR absorption, C/N ratio). The application of synchronous fluorescence and UV spectroscopy of aqueous samples has been proved to result in similar information about DOM composition as the characterization of isolated humic substances concerning the content of aromatic structures and the degree of humification.
我们研究的目的是测试在原始水样上应用耗时较短的光谱方法，以便在不进行任何样品制备的情况下获取有关溶解有机物（DOM）组成的信息。这些结果直接与从同一水样中分离出的溶解腐殖质（富里酸 - FA）的传统分离和表征程序的结果进行比较。通过紫外、荧光、傅里叶变换红外光谱和元素组成对富里酸进行表征。富里酸和原始水样的紫外吸光度和荧光行为遵循相同模式。与富里酸相比，水样的紫外吸光度较低，腐殖化指数（源自同步荧光光谱）约低15%是典型特征。我们计算了原始水样（紫外、同步荧光光谱）和分离出的富里酸（红外吸收、碳氮比）性质之间的线性关系。已证明对水样应用同步荧光和紫外光谱可获得与关于芳香结构含量和腐殖化程度的分离腐殖质表征类似的有关DOM组成的信息。
