大肠杆菌H7 fliC基因的序列多样性：对基于DNA的大肠杆菌O157:H7分型方案的意义。
Sequence diversity of the Escherichia coli H7 fliC genes: implication for a DNA-based typing scheme for E. coli O157:H7.
作者信息
Wang L, Rothemund D, Curd H, Reeves P R
机构信息
Department of Microbiology, The University of Sydney, Camperdown, New South Wales 2006, Australia.
出版信息
J Clin Microbiol. 2000 May;38(5):1786-90. doi: 10.1128/JCM.38.5.1786-1790.2000.
Abstract
Flagellar (H) antigens are mostly encoded by genes at the fliC locus in E. coli. We have sequenced 11 H7 fliC genes from Escherichia coli strains that belong to seven O serotypes. These sequences, together with those of nine other H7 fliC genes (from strains of three different O serotypes) sequenced recently (S. D. Reid, R. K. Selander, and T. S. Whittam, J. Bacteriol. 181:153-160, 1999), include 10 different sequences. The differences between these 10 sequences range from 0.06 to 3.12%. By comparison with other E. coli flagellin genes, we have identified primer length sequences specific for H7 genes in general and others specific for H7 genes of O157 and O55 strains: the specificity was confirmed by PCR testing the type strains for all 53 E. coli H types. We have previously identified genes specific for the E. coli O157 antigen, and use of the combination of O157- and H7-specific primers allows the sensitive and rapid detection of O157:H7 E. coli strains, which cause the majority of hemorrhagic colitis cases.
摘要
鞭毛（H）抗原大多由大肠杆菌中fliC位点的基因编码。我们对来自属于7种O血清型的大肠杆菌菌株的11个H7 fliC基因进行了测序。这些序列，连同最近测序的其他9个H7 fliC基因（来自三种不同O血清型的菌株）（S.D.里德、R.K.塞兰德和T.S.惠特姆，《细菌学杂志》181:153 - 160，1999），包括10种不同的序列。这10种序列之间的差异范围为0.06%至3.12%。通过与其他大肠杆菌鞭毛蛋白基因比较，我们确定了一般对H7基因特异的引物长度序列以及对O157和O55菌株的H7基因特异的其他序列：通过对所有53种大肠杆菌H型的模式菌株进行PCR检测证实了其特异性。我们之前已鉴定出对大肠杆菌O157抗原特异的基因，使用O157和H7特异性引物的组合能够灵敏且快速地检测出引起大多数出血性结肠炎病例的O157:H7大肠杆菌菌株。
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