Johnson J R, Stell A L
Medical Service, VA Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA.
J Clin Microbiol. 2001 Oct;39(10):3712-7. doi: 10.1128/JCM.39.10.3712-3717.2001.
A newly developed PCR-based assay for the H7 variant of the Escherichia coli flagellin gene, fliC, was 100% sensitive and specific in comparison with serology and probe hybridization. It revealed broad conservation of the H7 fliC variant among phylogenetically diverse lineages of extraintestinal pathogenic E. coli (ExPEC) and superseded serotyping for certain isolates with ambiguous or non-H7 serotyping results. The H7 primers functioned well when incorporated into a multiplex PCR assay for diverse virulence-associated genes of ExPEC.
一种新开发的基于聚合酶链反应（PCR）的检测大肠杆菌鞭毛蛋白基因fliC的H7变体的方法，与血清学和探针杂交相比，其敏感性和特异性均为100%。该方法揭示了H7 fliC变体在系统发育上不同的肠道外致病性大肠杆菌（ExPEC）谱系中具有广泛的保守性，并且对于某些血清型结果不明确或非H7血清型的分离株，该方法取代了血清分型。当将H7引物整合到用于ExPEC多种毒力相关基因的多重PCR检测中时，其效果良好。
