YCF1和YLL015w（BPT1）的产物协同作用，参与酿酒酵母中未结合胆红素的ATP依赖性液泡转运。
The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae.
作者信息
Petrovic S, Pascolo L, Gallo R, Cupelli F, Ostrow J D, Goffeau A, Tiribelli C, Bruschi C V
机构信息
Centro Studi Fegato (CSF), Department of BBCM, University of Trieste, Trieste, Italy.
出版信息
Yeast. 2000 Apr;16(6):561-71. doi: 10.1002/(SICI)1097-0061(200004)16:6<561::AID-YEA551>3.0.CO;2-L.
Since bilirubin-like pigments are present in the environment as degradation products of heme-containing proteins, yeast could have developed a detoxifying system to transport these compounds into their vacuoles. Vacuoles from Saccharomyces cerevisiae showed an ATP-dependent, saturative transport of unconjugated bilirubin (UCB) that was reduced by 60% and 40% in YCF1 and YLL015w-deleted cells, respectively; the double deletant showed no UCB uptake. Conversely, the transport of bile acids (taurocholate) was comparable in wild and deleted stains. These data identify YCF1 and YLL015w, named BPT1 (Bile Pigment Transporter), as the genes responsible for ATP-dependent UCB transport in yeast. Since YCF1 and YLL015w are rather homologous with multidrug resistant proteins (MRPs), they also suggest the involvement of this class of transporters in the ATP-dependent transport of unconjugated bilirubin.
由于胆红素样色素作为含血红素蛋白质的降解产物存在于环境中，酵母可能已形成一种解毒系统，将这些化合物转运至其液泡中。酿酒酵母的液泡显示出对未结合胆红素（UCB）的ATP依赖性饱和转运，在缺失YCF1和YLL015w的细胞中，该转运分别降低了60%和40%；双缺失株未显示出UCB摄取。相反，野生型和缺失株中胆汁酸（牛磺胆酸盐）的转运相当。这些数据确定YCF1和YLL015w（命名为BPT1，即胆汁色素转运蛋白）为酵母中负责ATP依赖性UCB转运的基因。由于YCF1和YLL015w与多药耐药蛋白（MRP）具有相当的同源性，它们还提示这类转运蛋白参与未结合胆红素的ATP依赖性转运。
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