Hillhouse E W, Burden J, Jones M T
Neuroendocrinology. 1975;17(1):1-11. doi: 10.1159/000122335.
The effect of various putative neurotransmitters upon the release of corticotrophin releasing hormone (CRH) was investigated using the hypothalamus of the rat in vitro. Acetylcholine (1-5 pg) caused a dose-dependent release of CRH which was antagonized by hexamethonium (1-10ng) and partially antagonized by atropine (300 pg). Neither noradrenaline, dopamine nor histamine had any effect on the basal secretion of CRH. Noradrenaline (10 ng), however, was able to inhibit the release of CRH in response to acetylcholine (3 pg) and this action of noradrenaline was reduced by phentolamine (100 ng), an alpha adrenergic blocking agent.
利用大鼠下丘脑体外实验，研究了各种假定神经递质对促肾上腺皮质激素释放激素（CRH）释放的影响。乙酰胆碱（1 - 5微克）引起CRH呈剂量依赖性释放，六甲铵（1 - 10纳克）可拮抗该作用，阿托品（300微克）可部分拮抗。去甲肾上腺素、多巴胺和组胺对CRH的基础分泌均无影响。然而，去甲肾上腺素（10纳克）能够抑制乙酰胆碱（3微克）引起的CRH释放，酚妥拉明（100纳克，一种α肾上腺素能阻断剂）可减弱去甲肾上腺素的这一作用。
