Kvetnanský R, Torda T, Jahnová E, Saleh N
Endocrinol Exp. 1975 Jun;9(2):79-86.
The activity of catecholamine degrading enzymes (catechol-O-methyltransferase--COMT and monoamine oxidase--MAO) in separated adrenal cortex and medulla was measured in rats stressed by a singel or repeated immobilization. In controls, the activity of both enzymes was higher in adrenal cortex than in adrenal medulla. After stress, the activity of COMT in adrenal cortex was significantly decreased, while that in adrenal medulla was significantly increased. Moreover, the animals repeatedly immobilized showed lower COMT activity in both parts of adrenals when compared to first time stressed rats. The activity of MAO was not remarkably changed, but was increased only in adrenal medulla when expressed per mg protein, the decrease of which resulted from acute stress. It was concluded that the findings of changed activity of catecholamine degrading enzymes, namely a decreased activity of COMT in both adrenal cortex and medulla in repeatedly stressed rats, might show a possible participation of these enzymes in increased catecholamine content in adrenals of repeatedly stressed animals.
在单次或重复固定应激的大鼠中，测定了分离的肾上腺皮质和髓质中儿茶酚胺降解酶（儿茶酚-O-甲基转移酶——COMT和单胺氧化酶——MAO）的活性。在对照组中，两种酶在肾上腺皮质中的活性均高于肾上腺髓质。应激后，肾上腺皮质中COMT的活性显著降低，而肾上腺髓质中的活性显著增加。此外，与首次应激的大鼠相比，重复固定的动物肾上腺两部分的COMT活性均较低。MAO的活性没有明显变化，但以每毫克蛋白质表示时仅在肾上腺髓质中增加，其降低是由急性应激导致的。得出的结论是，儿茶酚胺降解酶活性变化的结果，即重复应激大鼠肾上腺皮质和髓质中COMT活性降低，可能表明这些酶可能参与了重复应激动物肾上腺中儿茶酚胺含量的增加。
