Sorice M, Pittoni V, Griggi T, Losardo A, Leri O, Magno M S, Misasi R, Valesini G
Dipartimento di Medicina Sperimentale e Patologia, Clinica Medica 1, Università 'La Sapienza', Rome Italy.
Clin Exp Immunol. 2000 May;120(2):301-6. doi: 10.1046/j.1365-2249.2000.01206.x.
The antigen specificity of anti-phospholipid antibodies in infectious mononucleosis (IM) was studied using ELISA for the detection of anti-beta2-glycoprotein I (beta2-GPI), anti-annexin V, anti-protein S and anti-prothrombin antibodies and TLC immunostaining for the detection of anti-phospholipid antibodies. This technique enabled us to look at antibodies reacting to 'pure' phospholipid antigens in the absence of protein contamination. Sera from 46 patients with IM, 18 with systemic lupus erythematosus (SLE), 21 with primary anti-phospholipid antibody syndrome (PAPS), 50 with Helicobacter pylori infection and 30 healthy blood donors were tested. This study highlights anti-phospholipid antibodies in patients with IM as specific 'pure' anti-cardiolipin antibodies, while in PAPS and SLE patients anti-phosphatidylserine and anti-phosphatidylethanolamine antibodies were also found. This investigation also shows that the anti-cardiolipin antibodies found in IM can be present with anti-cofactor protein antibodies. The higher prevalence of anti-cofactor antibodies found in IM sera than in Helicobacter pylori sera may be due to the immunostimulatory effect and/or the polyclonal activation often observed in course of Epstein-Barr virus infection. However, anti-beta2-GPI and, to a lesser extent, anti-prothrombin antibodies occur with a significantly lower prevalence in IM than in PAPS patients. This finding suggests that these antibodies should be regarded as the expression of the broad autoimmune syndrome involving the phospholipid-binding plasma proteins.
采用酶联免疫吸附测定（ELISA）检测抗β2-糖蛋白I（β2-GPI）、抗膜联蛋白V、抗蛋白S和抗凝血酶原抗体，并通过薄层层析免疫染色检测抗磷脂抗体，研究传染性单核细胞增多症（IM）中抗磷脂抗体的抗原特异性。该技术使我们能够在不存在蛋白质污染的情况下观察与“纯”磷脂抗原反应的抗体。检测了46例IM患者、18例系统性红斑狼疮（SLE）患者、21例原发性抗磷脂抗体综合征（PAPS）患者、50例幽门螺杆菌感染患者和30名健康献血者的血清。本研究强调，IM患者中的抗磷脂抗体为特异性“纯”抗心磷脂抗体，而在PAPS和SLE患者中也发现了抗磷脂酰丝氨酸和抗磷脂酰乙醇胺抗体。该研究还表明，IM中发现的抗心磷脂抗体可能与抗辅助因子蛋白抗体同时存在。IM血清中抗辅助因子抗体的患病率高于幽门螺杆菌血清，这可能是由于在爱泼斯坦-巴尔病毒感染过程中经常观察到的免疫刺激作用和/或多克隆激活。然而，IM中抗β2-GPI抗体以及抗凝血酶原抗体的患病率明显低于PAPS患者。这一发现表明，这些抗体应被视为涉及磷脂结合血浆蛋白的广泛自身免疫综合征的表现。
