Clapcott S J, Teale A J, Kemp S J
School of Biological Sciences, Donnan Laboratories, University of Liverpool, Liverpool L69 7ZD, UK.
Parasite Immunol. 2000 May;22(5):259-63. doi: 10.1046/j.1365-3024.2000.00308.x.
Inbred strains of laboratory mice exhibit marked differences in survival time following infection with Trypanosoma congolense, the principal cause of trypanosomiasis in African livestock. The difference in survival time between the relatively resistant C57BL/6 J and more susceptible BALB/c inbred strains has been attributed to three quantitative trait loci (QTLs), Tir1, Tir2 and Tir3. In order to determine whether there was a parent-of-origin effect on this trait, four backcross populations derived from the C57BL/6 J and BALB/c parental strains were bred and inoculated with T. congolense. The two populations with F1 fathers and BALB/c mothers had a significantly greater overall survival rate than the two populations with BALB/c fathers and F1 mothers. This pattern of inheritance suggested the involvement of imprinted genes. Genotyping with markers at the three QTLs controlling susceptibility revealed that the difference in survival time was consistent with genomic imprinting of the QTL of largest effect, Tir1.
实验室小鼠的近交系在感染刚果锥虫后存活时间表现出显著差异，刚果锥虫是非洲家畜锥虫病的主要病因。相对抗性较强的C57BL/6 J近交系和易感性较高的BALB/c近交系之间存活时间的差异归因于三个数量性状基因座（QTL），即Tir1、Tir2和Tir3。为了确定该性状是否存在亲本来源效应，培育了来自C57BL/6 J和BALB/c亲本系的四个回交群体，并接种了刚果锥虫。具有F1代父本和BALB/c母本的两个群体的总体存活率显著高于具有BALB/c父本和F1代母本的两个群体。这种遗传模式表明存在印记基因的参与。用控制易感性的三个QTL的标记进行基因分型显示，存活时间的差异与效应最大的QTL即Tir1的基因组印记一致。
