关于在远交物种的实验杂交中检测印记数量性状基因座
On the detection of imprinted quantitative trait loci in experimental crosses of outbred species.
作者信息
de Koning Dirk-Jan, Bovenhuis Henk, van Arendonk Johan A M
机构信息
Animal Breeding and Genetics Group, Wageningen Institute of Animal Sciences, Wageningen University, 6700 AH Wageningen, The Netherlands.
出版信息
Genetics. 2002 Jun;161(2):931-8. doi: 10.1093/genetics/161.2.931.
In this article, the quantitative genetic aspects of imprinted genes and statistical properties of methods to detect imprinted QTL are studied. Different models to detect imprinted QTL and to distinguish between imprinted and Mendelian QTL were compared in a simulation study. Mendelian and imprinted QTL were simulated in an F2 design and analyzed under Mendelian and imprinting models. Mode of expression was evaluated against the H(0) of a Mendelian QTL as well as the H(0) of an imprinted QTL. It was shown that imprinted QTL might remain undetected when analyzing the genome with Mendelian models only. Compared to testing against a Mendelian QTL, using the H(0) of an imprinted QTL gave a higher proportion of correctly identified imprinted QTL, but also gave a higher proportion of false inference of imprinting for Mendelian QTL. When QTL were segregating in the founder lines, spurious detection of imprinting became more prominent under both tests, especially for designs with a small number of F1 sires.
本文研究了印记基因的数量遗传方面以及检测印记数量性状位点（QTL）方法的统计特性。在一项模拟研究中，比较了检测印记QTL以及区分印记QTL和孟德尔QTL的不同模型。在F2设计中模拟孟德尔和印记QTL，并在孟德尔模型和印记模型下进行分析。针对孟德尔QTL的原假设以及印记QTL的原假设评估表达模式。结果表明，仅使用孟德尔模型分析基因组时，印记QTL可能无法被检测到。与针对孟德尔QTL进行检验相比，使用印记QTL的原假设能正确鉴定出更高比例的印记QTL，但也会对孟德尔QTL产生更高比例的印记错误推断。当QTL在奠基系中分离时，在两种检验下印记的虚假检测都变得更加突出，尤其是对于具有少量F1父本的设计。
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