Morita K, Ono Y, Fukui H, Tomita S, Ueda Y, Terano A, Fujimori T
Departments of Pathology, Dokkyo University School of Medicine, Tochigi, Japan.
Pathol Int. 2000 Mar;50(3):219-23. doi: 10.1046/j.1440-1827.2000.01028.x.
Clarification of the pathogenic relationships existing among ovarian cystadenomas, tumors of low malignant potential (LMP) and various adenocarcinoma types, a series of 29 mucinous and 19 serous ovarian tumors including adenomas, LMP tumors and adenocarcinomas were examined. P53 protein was detected by the streptavidin-biotin method and point mutation of K-ras codon 12 was detected by polymerase chain reaction-restriction fragment length polymorphism analysis. P53 overexpression was observed more frequently in serous adenocarcinomas (5/8, 63%) than in mucinous adenocarcinomas (2/9, 22%) and was correlated with the malignant potential of serous tumors. Furthermore, the proportion of P53-positive cells was significantly higher in serous adenocarcinomas than in mucinous adenocarcinomas. P53 overexpression may therefore be closely related to the early events of carcinogenesis in serous tumors. Although mutation of the K-ras oncogene appears to be an important event in the early tumorigenesis of mucinous tumors, mutation of the K-ras oncogene in serous tumors may be dependent on morphology. Different complex pathways of oncogene and/or tumor suppressor gene abnormalities may be involved in the development of mucinous and serous adenocarcinomas.
为阐明卵巢囊腺瘤、低恶性潜能（LMP）肿瘤及各种腺癌类型之间存在的致病关系，我们检测了一系列包括腺瘤、LMP肿瘤和腺癌在内的29例黏液性和19例浆液性卵巢肿瘤。采用链霉亲和素-生物素法检测P53蛋白，通过聚合酶链反应-限制性片段长度多态性分析检测K-ras密码子12的点突变。浆液性腺癌（5/8，63%）中P53过表达的观察频率高于黏液性腺癌（2/9，22%），且与浆液性肿瘤的恶性潜能相关。此外，浆液性腺癌中P53阳性细胞的比例显著高于黏液性腺癌。因此，P53过表达可能与浆液性肿瘤发生的早期事件密切相关。虽然K-ras癌基因突变似乎是黏液性肿瘤早期肿瘤发生中的一个重要事件，但浆液性肿瘤中K-ras癌基因突变可能取决于形态学。黏液性和浆液性腺癌的发生可能涉及不同的癌基因和/或肿瘤抑制基因异常的复杂途径。
