Grau V, Gemsa D, Steiniger B, Garn H
Institute of Anatomy and Cell Biology, Philipps University Marburg; Institute of Immunology, Philipps University Marburg, Germany.
Scand J Immunol. 2000 May;51(5):435-40. doi: 10.1046/j.1365-3083.2000.00719.x.
During acute rejection of fully allogeneic rat renal allografts, few neutrophil granulocytes are detected, whereas an abundant infiltrate of macrophages and T lymphocytes becomes apparent. The mechanisms leading to this specific pattern of infiltration are not understood. We performed a sequential daily Northern blot analysis of the mRNA expression of the CC-chemokines MCP-1, MIP-1alpha and RANTES and of the CXC-chemokines GRO/KC and MIP-2 in rat renal isografts (LEW --> LEW, n = 1 per day) and allografts during acute rejection (DA --> LEW, n = 3 per day). MCP-1 gene expression strongly increased on days 3-4 after allotransplantation and returned to control levels on day 6. The expression of MIP-1alpha and RANTES continuously rose until day 3-4 and remained stable thereafter. Isografts displayed minor changes in CC-chemokine expression. In contrast to CC-chemokines, GRO/KC was expressed in low amounts during rejection and MIP-2 mRNA remained undetectable. In conclusion, the expression of the CC-chemokines MCP-1, MIP-1 and RANTES was clearly upregulated during rejection, whereas the mRNA of the CXC-chemokines MIP-2 and GRO/KC was not detected at all or remained at low levels. This pattern of chemokine gene expression is in good accordance with the predominant mononuclear leukocyte infiltrate in allografts.
在完全异基因大鼠肾移植急性排斥反应期间，几乎检测不到中性粒细胞，而巨噬细胞和T淋巴细胞的浸润却很明显。导致这种特定浸润模式的机制尚不清楚。我们对大鼠肾同基因移植（LEW→LEW，每天1只）和急性排斥反应期间的同种异体移植（DA→LEW，每天3只）进行了CC趋化因子MCP-1、MIP-1α和RANTES以及CXC趋化因子GRO/KC和MIP-2的mRNA表达的每日连续Northern印迹分析。同种异体移植后第3-4天，MCP-1基因表达强烈增加，第6天恢复到对照水平。MIP-1α和RANTES的表达持续上升至第3-4天，此后保持稳定。同基因移植的CC趋化因子表达变化较小。与CC趋化因子相反，GRO/KC在排斥反应期间表达量较低，且未检测到MIP-2 mRNA。总之，在排斥反应期间，CC趋化因子MCP-1、MIP-1和RANTES的表达明显上调，而CXC趋化因子MIP-2和GRO/KC的mRNA根本未检测到或保持在低水平。这种趋化因子基因表达模式与同种异体移植中主要的单核白细胞浸润情况高度一致。
