Ton H Y, Hughes R D, Silk D B, Williams R
Artif Organs. 1979 Feb;3(1):20-2. doi: 10.1111/j.1525-1594.1979.tb03799.x.
Platelets adhere to most foreign surfaces. As a result, polymers and albumin have been suggested as possible coatings to improve the blood compatibility of such surfaces. Amberlite XAD-7 has a high affinity for human serum albumin (HSA) and several protein-bound toxic metabolites. In the present study it is shown that HSA can be tightly bound to Amberlite XAD-7 without the use of chemical coupling agents. Optimal binding was achieved at pH 5.0 and the adsorption conforms to Langmuir's isotherm. Theoretical analysis of data and absence of a residual surface coating visible with scanning electron microscopy suggest a monolayer of albumin. The amount of HSA eluted from the resin under severe flow conditions was negligible (approximately 1%). Furthermore, in stirred batch studies with human plasma, the HSA coating did not decrease the adsorptive capacity of XAD-7 for bile acids and bilirubin.
血小板会黏附在大多数外来表面上。因此，有人提出聚合物和白蛋白可作为可能的涂层，以改善此类表面的血液相容性。Amberlite XAD - 7对人血清白蛋白（HSA）和几种与蛋白质结合的有毒代谢物具有高亲和力。在本研究中表明，无需使用化学偶联剂，HSA就能紧密结合到Amberlite XAD - 7上。在pH 5.0时实现了最佳结合，且吸附符合朗缪尔等温线。数据的理论分析以及扫描电子显微镜下未见残留表面涂层表明形成了白蛋白单分子层。在剧烈流动条件下从树脂上洗脱下来的HSA量可忽略不计（约1%）。此外，在用人血浆进行的搅拌批量研究中，HSA涂层并未降低XAD - 7对胆汁酸和胆红素的吸附能力。
