Brunner G, Holloway C J, Lösgen H
Artif Organs. 1979 Feb;3(1):27-30. doi: 10.1111/j.1525-1594.1979.tb03801.x.
A novel method of extracorporeal support for fulminant liver failure is reported whereby the most important detoxification processes of the liver are reproduced in an enzyme reactor. Most of the endogenous toxins involved in hepatic coma can be deactivated directly by conjugation with a hydrophilic residue such as glucuronic acid or glutathione, or by the neutralization of active groups through structural modification by methyl transfer. The enzymes responsible for these processes have been isolated and purified from rabbit liver, and covalently bound onto a hemocompatible form of agarose matrix. This system has been shown to be capable of catalyzing the desired reactions with endogenous toxins such as phenols and mercaptans in vitro, and phenols in rabbits in vivo.
报道了一种用于暴发性肝衰竭的新型体外支持方法，该方法通过在酶反应器中重现肝脏最重要的解毒过程来实现。参与肝昏迷的大多数内源性毒素可通过与亲水性残基（如葡萄糖醛酸或谷胱甘肽）结合，或通过甲基转移进行结构修饰来中和活性基团，从而直接失活。负责这些过程的酶已从兔肝脏中分离和纯化，并共价结合到具有血液相容性的琼脂糖基质上。该系统已被证明能够在体外催化与内源性毒素（如酚类和硫醇）以及在体内催化兔体内的酚类发生所需的反应。
