借助多核苷酸磷酸化酶对非放射性核糖核酸进行指纹分析。

Nucleic Acids Res. 1974 Jan;1(1):171-81. doi: 10.1093/nar/1.1.171.

We describe a method for obtaining radioactive fingerprints from nonradioactive ribonucleic acid. Fragments derived by T1 ribonuclease digestion of RNA are dephosphorylated with bacterial alkaline phosphatase. When these fragments are used as primers for the reaction of primer dependent polynucleotide phosphorylase with [alpha-(32)P]GDP in the presence of T1 ribonuclease the 3'-hydroxyl group of each fragment becomes phosphorylated. The degree of phosphorylation is reasonably uniform. The method has been applied to T1 ribonuclease digests of Escherichia coli tRNA(Met) (f); the oligonucleotides were further analyzed by spleen phosphodiesterase digestion. In a similar manner fingerprints of pancreatic ribonuclease digests of RNA can be obtained, when [alpha-(32)P]UDP, polynucleotide phosphorylase and pancreatic ribonuclease are used.

我们描述了一种从非放射性核糖核酸获得放射性指纹图谱的方法。通过T1核糖核酸酶消化RNA得到的片段用细菌碱性磷酸酶进行去磷酸化。当这些片段在T1核糖核酸酶存在的情况下用作引物依赖性多核苷酸磷酸化酶与[α-(32)P]GDP反应的引物时，每个片段的3'-羟基会被磷酸化。磷酸化程度相当均匀。该方法已应用于大肠杆菌tRNA(Met)(f)的T1核糖核酸酶消化物；寡核苷酸通过脾磷酸二酯酶消化进行进一步分析。以类似的方式，当使用[α-(32)P]UDP、多核苷酸磷酸化酶和胰核糖核酸酶时，可以获得RNA的胰核糖核酸酶消化物的指纹图谱。

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Annu Rev Biochem. 1973;42:303-28. doi: 10.1146/annurev.bi.42.070173.001511.

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Nucleic Acids Res. 1974 Jan;1(1):171-81. doi: 10.1093/nar/1.1.171.

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Fingerprinting nonradioactive ribonucleic acid with the aid of polynucleotide phosphorylase.

Nucleic Acids Res. 1974 Jan;1(1):171-81. doi: 10.1093/nar/1.1.171.

Sequence studies of nonradioactive Mycoplasma tRNA Phe with the aid of polynucleotide phosphorylase and polynucleotide kinase.

Nucleic Acids Res. 1974 Dec;1(12):1733-8. doi: 10.1093/nar/1.12.1733.

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J Biol Chem. 1974 Jan 10;249(1):149-51.

Polynucleotide phosphorylase can participate in decay of mRNA in Escherichia coli in the absence of ribonuclease II.

Mol Gen Genet. 1975 Sep 8;139(4):357-62. doi: 10.1007/BF00267975.

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J Bacteriol. 1977 Feb;129(2):651-7. doi: 10.1128/jb.129.2.651-657.1977.

RNA degradation in Escherichia coli regulated by 3' adenylation and 5' phosphorylation.

Nature. 1995 Mar 9;374(6518):180-3. doi: 10.1038/374180a0.

Oligonucleotide mapping of non-radioactive virus and messenger RNAs.

Nucleic Acids Res. 1977 Sep;4(9):2975-96. doi: 10.1093/nar/4.9.2975.

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J Bacteriol. 1976 Jul;127(1):637-43. doi: 10.1128/jb.127.1.637-643.1976.

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Nucleic Acids Res. 1976 Nov;3(11):2895-914. doi: 10.1093/nar/3.11.2895.

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Nucleic Acids Res. 1977 Jun;4(6):1957-78. doi: 10.1093/nar/4.6.1957.

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Nucleic Acids Res. 1977 Oct;4(10):3511-7. doi: 10.1093/nar/4.10.3511.

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Oligonucleotides as primers for polynucleotide phosphorylase.

J Biol Chem. 1960 Mar;235:738-50.

Use of polynucleotide kinase in fingerprinting non-radioactive nucleic acids.

J Mol Biol. 1969 Aug 14;43(3):607-17. doi: 10.1016/0022-2836(69)90362-3.

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J Mol Biol. 1965 Sep;13(2):373-98. doi: 10.1016/s0022-2836(65)80104-8.

New approach to the synthesis of polyribonucleotides of defined sequence.

Nature. 1971 Oct 22;233(5321):551-3. doi: 10.1038/233551a0.

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Nature. 1969 Sep 27;223(5213):1331-3. doi: 10.1038/2231331a0.

Nucleotide sequence of N-formyl-methionyl-transfer RNA.

Nature. 1968 Apr 20;218(5138):232-3. doi: 10.1038/218232a0.

Structure and function of phage RNA.

Annu Rev Biochem. 1973;42:303-28. doi: 10.1146/annurev.bi.42.070173.001511.

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Proc Natl Acad Sci U S A. 1973 Apr;70(4):1041-5. doi: 10.1073/pnas.70.4.1041.

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Eur J Biochem. 1969 Mar;8(2):244-55. doi: 10.1111/j.1432-1033.1969.tb00521.x.

Synthesis of trinucleoside diphosphates with polynucleotide phosphorylase.

Biochemistry. 1965 Aug;4(8):1561-7. doi: 10.1021/bi00884a015.

Fingerprinting nonradioactive ribonucleic acid with the aid of polynucleotide phosphorylase.

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借助多核苷酸磷酸化酶对非放射性核糖核酸进行指纹分析。

Fingerprinting nonradioactive ribonucleic acid with the aid of polynucleotide phosphorylase.

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