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借助多核苷酸磷酸化酶对非放射性核糖核酸进行指纹分析。

Fingerprinting nonradioactive ribonucleic acid with the aid of polynucleotide phosphorylase.

作者信息

Szeto K S, Söll D

出版信息

Nucleic Acids Res. 1974 Jan;1(1):171-81. doi: 10.1093/nar/1.1.171.

DOI:10.1093/nar/1.1.171
PMID:10793669
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343333/
Abstract

We describe a method for obtaining radioactive fingerprints from nonradioactive ribonucleic acid. Fragments derived by T1 ribonuclease digestion of RNA are dephosphorylated with bacterial alkaline phosphatase. When these fragments are used as primers for the reaction of primer dependent polynucleotide phosphorylase with [alpha-(32)P]GDP in the presence of T1 ribonuclease the 3'-hydroxyl group of each fragment becomes phosphorylated. The degree of phosphorylation is reasonably uniform. The method has been applied to T1 ribonuclease digests of Escherichia coli tRNA(Met) (f); the oligonucleotides were further analyzed by spleen phosphodiesterase digestion. In a similar manner fingerprints of pancreatic ribonuclease digests of RNA can be obtained, when [alpha-(32)P]UDP, polynucleotide phosphorylase and pancreatic ribonuclease are used.

摘要

我们描述了一种从非放射性核糖核酸获得放射性指纹图谱的方法。通过T1核糖核酸酶消化RNA得到的片段用细菌碱性磷酸酶进行去磷酸化。当这些片段在T1核糖核酸酶存在的情况下用作引物依赖性多核苷酸磷酸化酶与[α-(32)P]GDP反应的引物时，每个片段的3'-羟基会被磷酸化。磷酸化程度相当均匀。该方法已应用于大肠杆菌tRNA(Met)(f)的T1核糖核酸酶消化物；寡核苷酸通过脾磷酸二酯酶消化进行进一步分析。以类似的方式，当使用[α-(32)P]UDP、多核苷酸磷酸化酶和胰核糖核酸酶时，可以获得RNA的胰核糖核酸酶消化物的指纹图谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778a/343333/3804f6ac57a4/nar00510-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778a/343333/3804f6ac57a4/nar00510-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778a/343333/3804f6ac57a4/nar00510-0170-a.jpg

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本文引用的文献

1
Oligonucleotides as primers for polynucleotide phosphorylase.用作多核苷酸磷酸化酶引物的寡核苷酸。
J Biol Chem. 1960 Mar;235:738-50.
2
Use of polynucleotide kinase in fingerprinting non-radioactive nucleic acids.多核苷酸激酶在非放射性核酸指纹分析中的应用。
J Mol Biol. 1969 Aug 14;43(3):607-17. doi: 10.1016/0022-2836(69)90362-3.
3
A two-dimensional fractionation procedure for radioactive nucleotides.一种用于放射性核苷酸的二维分级分离程序。
寡核糖核苷酸的氚序列分析：一种用于分析部分蛇毒磷酸二酯酶消化产物的标记后与薄层色谱技术相结合的方法。
Nucleic Acids Res. 1974 Oct;1(10):1329-41. doi: 10.1093/nar/1.10.1329.
4
Sequence studies of nonradioactive Mycoplasma tRNA Phe with the aid of polynucleotide phosphorylase and polynucleotide kinase.借助多核苷酸磷酸化酶和多核苷酸激酶对非放射性支原体苯丙氨酸tRNA进行序列研究。
Nucleic Acids Res. 1974 Dec;1(12):1733-8. doi: 10.1093/nar/1.12.1733.
5
Sequence analysis of nonradioactive RNA fragments by periodate-phosphatase digestion and chemical tritium labeling: characterization of large oligonucleotides and oligonucleotides containing modified nucleosides.通过高碘酸盐-磷酸酶消化和化学氚标记对非放射性RNA片段进行序列分析：大寡核苷酸和含修饰核苷的寡核苷酸的表征
Nucleic Acids Res. 1974 Sep;1(9):1121-41. doi: 10.1093/nar/1.9.1121.
6
A double-labeling procedure for sequence analysis of picomole amounts of nonradioactive RNA fragments.一种用于皮摩尔量非放射性RNA片段序列分析的双标记方法。
Nucleic Acids Res. 1976 Nov;3(11):2895-914. doi: 10.1093/nar/3.11.2895.
7
Use of specific endonuclease cleavage in RNA sequencing.特定核酸内切酶切割在RNA测序中的应用。
Nucleic Acids Res. 1977 Jun;4(6):1957-78. doi: 10.1093/nar/4.6.1957.
8
A rapid method for preparation of calf spleen exonuclease.一种制备小牛脾外切核酸酶的快速方法。
Nucleic Acids Res. 1977 Oct;4(10):3511-7. doi: 10.1093/nar/4.10.3511.
J Mol Biol. 1965 Sep;13(2):373-98. doi: 10.1016/s0022-2836(65)80104-8.
4
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Nature. 1971 Oct 22;233(5321):551-3. doi: 10.1038/233551a0.
5
Structure of transfer RNA. Evidence for interaction between two non-adjacent nucleotide residues in tRNA from Escherichia coli.转运RNA的结构。大肠杆菌转运RNA中两个不相邻核苷酸残基之间相互作用的证据。
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6
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7
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Annu Rev Biochem. 1973;42:303-28. doi: 10.1146/annurev.bi.42.070173.001511.
8
Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs.几种真核细胞质起始转运核糖核酸中不存在G-T-假尿嘧啶-C-G(A)序列。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1041-5. doi: 10.1073/pnas.70.4.1041.
9
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10
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Biochemistry. 1965 Aug;4(8):1561-7. doi: 10.1021/bi00884a015.