de Recondo A M, Londos-Gagliardi D, Aubel-Sadron G
Nucleic Acids Res. 1974 Oct;1(10):1233-47. doi: 10.1093/nar/1.10.1233.
The template activity of Cancer pagurus DNA and its two components (poly d(A-T) and main component) in response to a DNA polymerase purified from regenerating rat liver has been studied and compared to the results previously obtained with synthetic templates. In the double-stranded native state, whole crab DNA and the main component were poor templates. Their replication was increased by thermal denaturation and inhibited by actinomycin. Like the synthetic copolymer poly[d(A-T).d(T-A)], native crab poly d(A-T) could be copied and its duplication was not inhibited by actinomycin. The structural difference between native poly d(A-T) Form I, isolated on a density gradient, and partially renatured poly d(A-T) Form II, isolated on hydroxylapatite, resulted in a modification of their template activity. The kinetic studies of [(3)H] dGMP and [(3)H] dAMP incorporation confirmed the importance of single-stranded regions (particulary dC regions) in the initiation of the in vitro duplication.
研究了黄道蟹DNA及其两个组分（聚d（A-T）和主要组分）对从再生大鼠肝脏中纯化的DNA聚合酶的模板活性，并将其与先前用合成模板获得的结果进行了比较。在双链天然状态下，完整的蟹DNA和主要组分是较差的模板。热变性可增加它们的复制，而放线菌素可抑制其复制。与合成共聚物聚[d（A-T）·d（T-A）]一样，天然蟹聚d（A-T）可以被复制，并且其复制不受放线菌素抑制。在密度梯度上分离得到的天然聚d（A-T）I型与在羟基磷灰石上分离得到的部分复性聚d（A-T）II型之间的结构差异导致了它们模板活性的改变。[（3）H]dGMP和[（3）H]dAMP掺入的动力学研究证实了单链区域（特别是dC区域）在体外复制起始中的重要性。
