使用外源性DNA聚合酶对塑料包埋组织超薄切片中的DNA复制进行放射自显影演示。
Autoradiographic demonstration of DNA replication in ultrathin sections of plastic-embedded tissues using an exogeneous DNA polymerase.
作者信息
Geuskens M, de Recondo A M, Chevaillier P
出版信息
Chromosoma. 1975 Sep 26;52(2):175-88. doi: 10.1007/BF00326266.
PMID:1175463
Abstract
A DNA-dependent DNA polymerase isolated from regenerating rat liver can mediate the incorporation of tritiated nucleoside triphosphates into acid-insoluble polydeoxyribonucleotides using the DNA contained in ultrathin sections of glycol methacrylate-embedded crab testis as initiator-template. (Summary see p. 186).
摘要
从再生大鼠肝脏中分离出的一种依赖DNA的DNA聚合酶，能够以乙二醇甲基丙烯酸酯包埋的蟹睾丸超薄切片中所含的DNA为引发剂模板，介导将氚标记的核苷三磷酸掺入酸不溶性多脱氧核糖核苷酸中。（摘要见第186页）
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