Dornan V, Cook E A, Carey N H
Nucleic Acids Res. 1974 Nov;1(11):1561-71. doi: 10.1093/nar/1.11.1561.
Total RNA from hen oviduct has been hydrolysed with a mixture of T(1) and pancreatic ribonucleases. Poly(A) tracts in the digestion product have been isolated by binding to oligo(dT) cellulose. Of the four major ribonucleotides, the product has been shown to contain only adenylic acid. When separated on polyacrylamide gels, the poly(A) gave two peaks corresponding to average apparent lengths of 270-280 and 540-550 nucleotides.
来自母鸡输卵管的总RNA已用T(1)和胰核糖核酸酶混合物进行水解。消化产物中的聚腺苷酸（Poly(A)）片段通过与寡聚（dT）纤维素结合而被分离出来。在四种主要核糖核苷酸中，该产物已被证明仅含有腺苷酸。当在聚丙烯酰胺凝胶上分离时，聚腺苷酸产生了两个峰，分别对应于平均表观长度为270 - 280个核苷酸和540 - 550个核苷酸。
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