人前列腺癌细胞（MA - 160）含多聚腺苷酸（poly(A)）RNA的修饰核苷酸成分。

The modified nucleotide constituents of human prostatic cancer cell (MA-160) poly(A)-containing RNA.

作者信息

Nichols J L, Welder L

出版信息

Biochim Biophys Acta. 1980 Jun 27;608(1):1-18. doi: 10.1016/0005-2787(80)90128-8.

Abstract

Cytoplasmic poly A(+) RNA from human prostatic cancer cells grown in the presence of 32P was isolated by affinity chromatography on columns of oligo(dT)-cellulose. The RNA was digested with RNAase T2 and the products of digestion were fractionated by two-dimensional electrophoresis. The resulting autoradiograms revealed the presence of many different cap groups as well as two internal modified nucleotide components. 19 different type 1 and type 2 'cap' groups were identified. The internal modified nucleotides were N6-methyl adenosine and a 2'-O-methyl nucleotide possessing an unusual modified base.

摘要

通过在寡聚（dT）-纤维素柱上进行亲和层析，从在32P存在下生长的人前列腺癌细胞中分离出细胞质多聚A（+）RNA。用RNA酶T2消化该RNA，并通过二维电泳对消化产物进行分级分离。所得的放射自显影片显示存在许多不同的帽结构基团以及两种内部修饰的核苷酸成分。鉴定出19种不同类型的1型和2型“帽”结构基团。内部修饰的核苷酸是N6-甲基腺苷和一种具有异常修饰碱基的2'-O-甲基核苷酸。