Pevzner E, Ehrenberg B
Department of Physics, Bar Ilan University, Ramat Gan, Israel.
Spectrochim Acta A Mol Biomol Spectrosc. 2000 Mar;56(4):637-51. doi: 10.1016/s1386-1425(99)00164-x.
Metallochromic indicators, whose spectral properties are changed in the presence of metal cations, are used mainly in biological studies to monitor Ca2+ and Mg2+ ions. Antipyrylazo III is such indicator, employed for mid-range Ca2+ concentrations (10-1000 microM). The stoichiometry of the interactions of antipyrylazo III with Ca2+, Mg2+, Ba2+, Sr2+ and Zn2+ ions and the relevant binding constants were studied by principal component analysis (PCA) of the absorption spectral changes. The resonance Raman spectra of the above systems were measured as well, and the resolved Raman spectra of the various species were calculated and assigned. The vibrational spectra are more featured, more characteristic of the binding ions and exhibit stronger relative spectral changes upon binding the cations. The basis sets of Raman spectra could thus be used as an analytical tool for these divalent metallic cations.
金属变色指示剂在金属阳离子存在下其光谱性质会发生变化，主要用于生物学研究中监测Ca2+和Mg2+离子。安替比拉宗III就是这样一种指示剂，用于中等浓度的Ca2+（10 - 1000微摩尔）。通过对吸收光谱变化进行主成分分析（PCA），研究了安替比拉宗III与Ca2+、Mg2+、Ba2+、Sr2+和Zn2+离子相互作用的化学计量关系以及相关的结合常数。还测量了上述体系的共振拉曼光谱，并计算和归属了各种物种的分辨拉曼光谱。振动光谱更具特征，更能体现结合离子的特性，并且在结合阳离子时表现出更强的相对光谱变化。因此，拉曼光谱的基组可以用作这些二价金属阳离子的分析工具。
