金属铬指示剂染料安替比拉宗III与钙平衡反应的体外校准。
In vitro calibration of the equilibrium reactions of the metallochromic indicator dye antipyrylazo III with calcium.
作者信息
Hollingworth S, Aldrich R W, Baylor S M
出版信息
Biophys J. 1987 Mar;51(3):383-93. doi: 10.1016/S0006-3495(87)83360-X.
Abstract
The equilibrium reactions of the metallochromic indicator dye Antipyrylazo III with calcium at physiological ionic strength have been investigated spectrophotometrically. Dye absorbance as a function of wavelength was measured at various total dye and calcium concentrations. Analysis of the absorbance spectra indicated that at pH 6.9 at least three calcium:dye complexes form, with 1:1, 1:2, and possibly 2:2 stoichiometries. The dissociation constant and the changes in dye extinction coefficients on formation of the 1:2 complex, the main complex which forms when Antipyrylazo III is used to study cytoplasmic calcium transients, have been characterized.
摘要
采用分光光度法研究了金属铬指示剂染料安替比拉佐III在生理离子强度下与钙的平衡反应。在不同的总染料和钙浓度下，测量了染料吸光度随波长的变化。吸光度光谱分析表明，在pH 6.9时至少形成三种钙:染料络合物，化学计量比分别为1:1、1:2，可能还有2:2。当使用安替比拉佐III研究细胞质钙瞬变时形成的主要络合物1:2络合物的解离常数和染料消光系数的变化已得到表征。
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Stoichiometry of the reactions of calcium with the metallochromic indicator dyes antipyrylazo III and arsenazo III.钙与金属显色指示剂染料安替比拉宗III和偶氮胂III反应的化学计量学
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