Tenti P, Vesentini N, Rondo Spaudo M, Zappatore R, Migliora P, Carnevali L, Ranzani G N
Department of Human and Hereditary Pathology, Anatomic Pathology Section, University of Pavia and Instituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Italy.
Cancer Epidemiol Biomarkers Prev. 2000 Apr;9(4):435-8.
A case-control study was performed to investigate the risk of cervical cancer associated with p53 polymorphism at codon 72, encoding either arginine or proline. It has been recently suggested that the arginine isoform increases the susceptibility to invasive cervical cancer; however, data remain controversial. The polymorphism was examined by both allele-specific PCR and RFLP analysis in 101 patients with primary cervical cancer and in 140 healthy women of the same age and from the same geographical area. The distribution of p53 genotypes in cervical cancer patients and in controls was not significantly different (P = 0.445), and homozygosity for arginine at residue 72 was not associated with an increased risk for cervical cancer (odds ratio, 0.81; 95% confidence interval, 0.47-1.42; P = 0.52). Similarly, different genotype distribution and increased risk were not observed when patients versus controls were analyzed according to human papillomavirus status and cancer histotype. Therefore, no evidence of association between homozygosity for p53 arginine and cervical cancer was found in our population sample.
开展了一项病例对照研究，以调查编码精氨酸或脯氨酸的密码子72处p53基因多态性与宫颈癌风险的相关性。最近有研究表明，精氨酸异构体增加了浸润性宫颈癌的易感性；然而，数据仍存在争议。采用等位基因特异性PCR和限制性片段长度多态性分析，对101例原发性宫颈癌患者和140例来自相同地理区域的同龄健康女性进行了该多态性检测。宫颈癌患者和对照组中p53基因型的分布无显著差异（P = 0.445），72位残基的精氨酸纯合性与宫颈癌风险增加无关（比值比，0.81；95%置信区间，0.47 - 1.42；P = 0.52）。同样，根据人乳头瘤病毒状态和癌症组织学类型对患者与对照组进行分析时，未观察到不同的基因型分布和风险增加。因此，在我们的人群样本中，未发现p53精氨酸纯合性与宫颈癌之间存在关联的证据。
